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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts

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Author(s):
Franca, Maria G. A. [1] ; Cavalheiro, Alberto J. [2] ; Silva, Maria G. V. [1]
Total Authors: 3
Affiliation:
[1] Univ Fed Ceara, Dept Quim Organ & Inorgan, Dept Quim Analit & Fis Quim, BR-60455970 Fortaleza, Ceara - Brazil
[2] Univ Estadual Paulista, Inst Quim, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 31, n. 1, p. 32-39, FEB 2021.
Web of Science Citations: 0
Abstract

A reversed-phase analytical method was developed and validated by using a combination of high-performance liquid chromatography with diode array detection and ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry systems to dereplicate 46 compounds in samples prepared by mixing extracts from various organs of different Senna species, Fabaceae. The pre-treatment of the sample by solid phase extraction was also investigated. The results obtained showed that the clean-up of the plant extract produces relevant changes in its chemical profile, resulting in false fingerprints. The efficiency of the method was applying to compare the chemical profile of the extracts of seven different Senna species: S. cearensis Afr. Fern, S. macranthera (Collad.) H.S.Irwin \& Bameby, S. obtusffolia (L.) H.S.Irwin \& Bameby, S. reticulata (Willd.) H.S.Irwin \& Barneby, S. spectabilis var. excelsa (DC.) H.S.Irwin \& Barneby, S. splendida (Vogel) H.S.Irwin \& Barneby, and S. trachypus (Benth.) H.S.Irwin \& Barneby. These results suggest that the comprehensive developed method can be applied to the study and dereplication of the chemical composition of other Senna species, preferentially without clean-up of the samples. (AU)

FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
Grantee:Vanderlan da Silva Bolzani
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants