hysicochemical and biological properties of new tricalcium silicate-based repair material doped with fluoride ions and zirconium oxide as radiopacifie

Author(s)

Campi, Livia Bueno ^[1] ; Esteves Torres, Fernanda Ferrari ^[1] ; Rodrigues, Elisandra Marcia ^[1] ; Guerreiro-Tanomaru, Juliane Maria ^[1] ; Tanomaru-Filho, Mario ^[1]

Affiliation

^[1] Sao Paulo State Univ, Sch Dent, Dept Restorat Dent, UNESP, Araraquara, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 110, n. 4 NOV 2021.
• Web of Science Citations: 0

Abstract

This study evaluated the physicochemical and biological properties of novel reparative materials composed of pure tricalcium silicate (Ca3SiO5), Ca3SiO5 doped with fluoride ions (Ca3SiO5-F) and their association with ZrO2 (Ca3SiO5 + ZrO2, Ca3SiO5-F + ZrO2), in comparison with Biodentine (BIO). Setting time radiopacity, pH, solubility, and dimensional change were evaluated based on ISO 6876 Standard. Volumetric change and flow/filling were assessed by microcomputed tomography (micro-CT). Biological properties were evaluated by the MTT assay 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), cell migration, alkaline phosphatase activity (ALP), and Alizarin Red Staining (ARS) assays. Statistical analysis was performed by ANOVA, Tukey, or Bonferroni tests (alpha = .05). Ca3SiO5-F + ZrO2 had higher radiopacity, shorter setting time, and lower solubility and volumetric loss than BIO (p < .05). Ca3SiO5-F + ZrO2 had flow and filling capacity similar to BIO (p > .05). All the cements evaluated had an alkaline pH. Ca3SiO5-F + ZrO2 demonstrated cell viability similar to negative control (p > .05), increase in ALP activity in 7 days, mineralized nodule production in 21 days and repair capacity according to cell migration. In conclusion, Ca3SiO5-F + ZrO2 had adequate setting time, radiopacity, solubility, and dimensional change. This material presented low volumetric change besides flow and filling capacity in micro-CT assessment. In addition, Ca3SiO5-F + ZrO2 was biocompatible and bioactive, suggesting its use as reparative material. (AU)

• FAPESP's process: 18/25364-9 - Physicochemical and biological characteristics of new bioceramic reparative materials and tricalcium silicate doped with fluorine ions cements
• Grantee: Lívia Bueno Campi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/14305-9 - Evaluation of new substances for root canal irrigation and calcium silicate-based endodontic sealers
• Grantee: Juliane Maria Guerreiro Tanomaru
• Support Opportunities: Regular Research Grants