Atypical Prolonged Viral Shedding With Intra-Host SARS-CoV-2 Evolution in a Mildly Affected Symptomatic Patient

Author(s):Show less -

Cunha, Marielton dos Passos ^[1] ; Vilela, Ana Paula Pessoa ^[1] ; Molina, Camila Vieira ^[1] ; Acuna, Stephanie Maia ^[1] ; Muxel, Sandra Marcia ^{[2, 1]} ; Barroso, Vinicius de Morais ^{[2, 1]} ; Baroni, Sabrina ^[1] ; Gomes de Oliveira, Lilian ^[1] ; Angelo, Yan de Souza ^[1] ; Peron, Jean Pierre Schatzmann ^{[2, 1]} ; Goes, Luiz Gustavo Bentim ^{[2, 1]} ; Campos, Angelica Cristine de Almeida ^[1] ; Minoprio, Paola ^{[3, 1]}

Affiliation

^[1] Sci Platform Pasteur USP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[3] Inst Pasteur, Paris - France

• Document type: Journal article
• Source: FRONTIERS IN MEDICINE; v. 8, NOV 26 2021.
• Web of Science Citations: 0

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is caused by a respiratory virus with a wide range of manifestations, varying from asymptomatic to fatal cases, with a generally short outcome. However, some individuals present long-term viral shedding. We monitored 38 individuals who were mildly affected by the SARS-CoV-2 infection. Out of the total studied population, three (7.9%) showed atypical events regarding the duration of positivity for viral RNA detection. In one of these atypical cases, a previously HIV-positive male patient presented a SARS-CoV-2 RNA shedding and subgenomic RNA (sgRNA) detected from the upper respiratory tract, respectively, for 232 and 224 days after the onset of the symptoms. The SARS-CoV-2 B.1.1.28 lineage, one of the most prevalent in Brazil in 2020, was identified in this patient in three serial samples. Interestingly, the genomic analyses performed throughout the infectious process showed an increase in the genetic diversity of the B.1.1.28 lineage within the host itself, with viral clearance occurring naturally, without any intervention measures to control the infection. Contrasting widely spread current knowledge, our results indicate that potentially infectious SARS-CoV-2 virus might be shed by much longer periods by some infected patients. This data call attention to better adapted non-pharmacological measures and clinical discharge of patients aiming at preventing the spread of SARS-CoV-2 to the population. (AU)

• FAPESP's process: 19/20708-4 - Impact of Trypanosoma vivax infections on blood brain barrier crossing / disruption in murine systems
• Grantee: Ana Paula Pessoa Vilela
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/11612-0 - Investigation of the antifungal activity and mechanism of action of 2-aryloxazolines derivatives on Candida albicans
• Grantee: Vinicius de Morais Barroso
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/27131-9 - Integrated strategy for the study of infectious agents causing emerging and/or neglected diseases transmitted by vectors of global impact
• Grantee: Paola Marcella Camargo Minoprio
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 19/24518-5 - Integrated strategy for the study of infectious agents causing emerging and/or neglected diseases transmitted by national and regional impact vectors
• Grantee: Marielton dos Passos Cunha
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/23519-2 - Analysis of role of miRNAs and transcription factors on the regulation of gene expression of Leishmania amazonensis infected murine macrophages
• Grantee: Stephanie Maia Acuna
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/01487-4 - Survey of respiratory viruses (Influenza, Paramyxovirus, Coronavirus and Bocavirus) in captive and wild nonhuman primates from metropolitan area of São Paulo city
• Grantee: Camila Vieira Molina
• Support type: Scholarships in Brazil - Doctorate