| Full text | |
| Author(s): |
Materon, Elsa M.
[1, 2]
;
Shimizu, Flavio M.
[1, 3]
;
dos Santos, Kevin Figueiredo
[1]
;
Nascimento, Gustavo F.
[1]
;
Geraldo, Vananelia P. N.
[1]
;
Oliveira Jr, Osvaldo N.
;
Faria, Ronaldo C.
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Chem Dept, CP 676, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Gleb Wataghin Inst Phys IFGW, Dept Appl Phys, UNICAMP, BR-13083859 Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | BIOMEDICINE & PHARMACOTHERAPY; v. 145, JAN 2022. |
| Web of Science Citations: | 0 |
| Abstract | |
Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and investigated their interaction with the anticancer drugs cisplatin (CDDP) and doxorubicin (DOX). We found that both DOX and CDDP expand and affect the elasticity of MIX monolayers, but these effects are hindered when glutathione-s-transferase (GST) and its cofactor glutathione (GSH) are incorporated. Changes are induced by DOX or CDDP on the polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS) data for MIX/GST/GSH monolayers, thus denoting some degree of interaction that is not sufficient to alter the monolayer mechanical properties. Overall, the results presented here give support to the hypothesis of the inactivation of DOX and CDDP by GST and point to possible directions to detect and fight drug resistance. (AU) | |
| FAPESP's process: | 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue |
| Grantee: | Flavio Makoto Shimizu |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases |
| Grantee: | Ronaldo Censi Faria |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/00991-5 - Study of Detoxification Enzymes Interaction with chemotherapeutic drugs and its relationship with chemoresistance |
| Grantee: | Elsa María Materón Vásques |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |