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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant Production and Characterization of a New Toxin from Cryptops iheringi Centipede Venom Revealed by Proteome and Transcriptome Analysis

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Author(s):
Caetano, Lhiri Hanna De Lucca [1] ; Nishiyama-Jr, Milton Yutaka ; Tavora, Bianca de Carvalho Lins Fernandes [1] ; de Oliveira, Ursula Castro [2] ; Junqueira-de-Azevedo, Inacio de Loiola Meirelles [2] ; Faquim-Mauro, Eliana L. [1] ; Magalhaes, Geraldo Santana [1]
Total Authors: 7
Affiliation:
[1] Inst Butantan, Lab Imunopatol, BR-05503900 Sao Paulo - Brazil
[2] Nishiyama-Jr, Jr., Milton Yutaka, Inst Butantan, Lab Toxinol Aplicada, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TOXINS; v. 13, n. 12 DEC 2021.
Web of Science Citations: 0
Abstract

Among the Chilopoda class of centipede, the Cryptops genus is one of the most associated with envenomation in humans in the metropolitan region of the state of Sao Paulo. To date, there is no study in the literature about the toxins present in its venom. Thus, in this work, a transcriptomic characterization of the Cryptops iheringi venom gland, as well as a proteomic analysis of its venom, were performed to obtain a toxin profile of this species. These methods indicated that 57.9% of the sequences showed to be putative toxins unknown in public databases; among them, we pointed out a novel putative toxin named Cryptoxin-1. The recombinant form of this new toxin was able to promote edema in mice footpads with massive neutrophils infiltration, linking this toxin to envenomation symptoms observed in accidents with humans. Our findings may elucidate the role of this toxin in the venom, as well as the possibility to explore other proteins found in this work. (AU)

FAPESP's process: 17/13812-4 - Transcriptome, cloning, expression and evaluation of the biological activities of the main toxins of Cryptops iheringi venom
Grantee:Lhiri Hanna de Lucca Caetano
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/16999-8 - Transcriptomic analysis of venom gland Cryptops iheringi, heterologous expression of major toxins and study of their biological activities
Grantee:Geraldo Santana Magalhães
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC