ngestion of orange juice prevents hyperglycemia and increases plasma miR-375 expressio

Author(s)

Quintanilha, Bruna J. ^{[1, 2]} ; Chaves, Daniela F. S. ^[3] ; Brasili, Elisa ^[4] ; Correa, Telma A. F. ^{[2, 3]} ; Capetini, Vinicius Cooper ^{[1, 2]} ; Ferreira, Frederico Moraes ^[5] ; Castro, Inar A. ^{[2, 3]} ; Hassimotto, Neuza M. A. ^{[2, 3]} ; Rogero, Marcelo M. ^{[1, 2]} ; Lajolo, Franco M. ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Sao Paulo - Brazil

^[2] CEPID FAPESP Res Innovat & Disseminat Ctr Sao Pau, Food Res Ctr FoRC, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Food Sci & Expt Nutr, Sch Pharmaceut Sci, Sao Paulo - Brazil

^[4] Sapienza Univ Rome, Dept Environm Biol, Rome - Italy

^[5] Univ Sao Paulo, Div Pathol, Sch Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: CLINICAL NUTRITION ESPEN; v. 47, p. 240-245, FEB 2022.
• Web of Science Citations: 0

Abstract

Background \& aims: The intake of high-fat, high-carbohydrate (HFHC) meals is associated with an increased risk of type 2 diabetes. There is evidence that the association of orange juice to a HFHC meal can modulate the expression of microRNAs (miRNAs) linked to pancreatic beta-cell function such as miR-375. We evaluated the effect of a commercial orange juice intake with HFHC meal on plasma miRNAs expression in twelve healthy subjects in a crossover design study. Methods: Subjects ingested water, orange juice, or an isocaloric beverage along with a 1037 kcal HFHC meal. Blood glucose and miRNAs were evaluated at baseline and 1, 3, and 5 h after the intake. Results: The area under the curve (AUC) for glycemia after ingestion of HFHC + orange juice did not differ from ingestion of HPHC + glucose or HFHC + water. However, the AUC was higher in HFHC meal + glucose compared to HFHC meal + water (p = 0.034). Glucose and insulin concentrations were significantly higher in HFHC meal + glucose group after 1 h, when compared with other groups and times (p < 0.001). There was an increase in plasma miR-375 expression after 3 h of ingestion of HFHC + orange juice versus water (p = 0.026), and a decrease in plasma miR-205-5p expression after HFHC meal + glucose versus water (p = 0.023). Conclusions: A single HFHC meal + orange juice modulated plasma miR-375 expression, which is a biomarker of pancreatic beta-cell function, and contributed to preventing hyperglycemia. (C) 2021 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/22864-0 - Biomarkers identification of dietary polyphenols intake using metabolome and proteome approaches
• Grantee: Daniela Fojo Seixas Chaves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC