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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

iagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protei

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Author(s):
Soares, Juliana C. [1] ; Soares, Andrey C. [2] ; Angelim, Monara Kaelle S. C. [3] ; Proenca-Modena, Jose Luiz [4, 3] ; Moraes-Vieira, Pedro M. [4, 3, 5] ; Mattoso, Luiz H. C. [2] ; Oliveira Jr, Osvaldo N.
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13566590 Sao Carlos - Brazil
[2] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Expt Med Res Cluster EMRC, BR-13083862 Campinas, SP - Brazil
[5] Univ Estadual Campinas, Obes & Comorbil Res Ctr OCRC, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Talanta; v. 239, MAR 1 2022.
Web of Science Citations: 1
Abstract

Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10-20 g/mL to 10-14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C-H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. (AU)

FAPESP's process: 18/18953-8 - Nanostructured films applied in microfluidic biosensors to mastitis detection
Grantee:Andrey Coatrini Soares
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 20/08744-2 - Risk factors associated with greater severity to COVID-19 and mapping of metabolic pathways required for the anti-SARSCoV-2 response
Grantee:Monara Kaélle Sérvulo Cruz Angelim
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 20/04579-7 - Study of the risk factors associated with greater severity to COVID-19 and mapping of the metabolic pathways required for the anti-SARS-CoV-2 response
Grantee:Pedro Manoel Mendes de Moraes Vieira
Support Opportunities: Regular Research Grants