Interlaboratory exercise for the analysis of carotenoids and related compounds in dried mango fruit (Mangifera indica L.)

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Villacis-Chiriboga, Jose ; Jacobs, Griet ; Van Camp, John ; Elst, Kathy ; Ruales, Jenny ; Marcillo-Parra, Veronica ; Bohm, Volker ; Bunea, Andrea ; Cirlini, Martina ; Craft, Neal ; De Meulenaer, Bruno ; Dias, M. Graca ; Lazzarino, Giacomo ; Melendez-Martinez, Antonio J. ; Versloot, Pieter ; Mercadante, Adriana Z. ; Olmedilla-Alonso, Begona ; Ortiz-Ulloa, Johana ; Stinco, Carla M. ; Voorspoels, Stefan

• Document type: Journal article
• Source: Journal of Food Composition and Analysis; v. 111, p. 9-pg., 2022-08-01.

Abstract

An interlaboratory comparison was done for the analysis of carotenoids in freeze-dried mango. The study was performed from July to September 2018. Mango fruit was freeze-dried, homogenized, and packaged under vacuum conditions in portions of 6 g (test sample). Two test samples were sent to the participating laboratories for analysis. Laboratory results were rated using Z-scores in accordance with ISO 13528 and ISO 17043. The standard deviation for proficiency assessment (also called target standard deviation) was determined using a modified Horwitz function and varied between 10% and 25%, depending on the analyte. Out of 14 laboratories from 10 different countries, 9 laboratories (64%) obtained a satisfactory performance (Z & LE; 2) for the analysis of 0-carotene. While for 7 laboratories that analyzed a-carotene, (9Z) -0-carotene, 0-cryptoxanthin, and zeaxanthin, 4 laboratories (57%) obtained a satisfactory performance. However, only 2 laboratories out of 7 (29%) obtained a satisfactory performance for lutein. Based on the comparability of the analytical results, this study concludes that freeze-dried mango pulp can be used as a reference material for the analysis of a and 0-carotene, (9Z)-0-carotene, 0-cryptoxanthin, and zeaxanthin by applying different analytical procedures for their extraction and quantification. (AU)

• FAPESP's process: 18/23752-1 - Multi0user equipment approved in grant 2013/07914-8: high-speed liquid chromatography coupled to arrangement of diodes, fluorescence and masses
• Grantee: Adriana Zerlotti Mercadante
• Support Opportunities: Multi-user Equipment Program