| Full text | |
| Author(s): |
Dionizio, Bruna Soares
;
Silva Rabelo, Camila Abreu B.
;
Ramos de Jesus, Hugo Cesar
;
Amancio Varesche, Maria Bernadete
;
Ferreira de Souza, Dulce Helena
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Applied Biochemistry and Biotechnology; v. 194, n. 7, p. 22-pg., 2022-03-29. |
| Abstract | |
The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes. This study was undertaken to explore the applicability of a new extract with the enzymes (Lac(mix)) isolated from Chaetomium cupreum for SCB pretreatment. Lac(mix) was more active at pH of 2.2 to 4 and 50 to 60 degrees C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lac(mix) (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design. The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL(- 1) laccases, and 2.44 h resulting in 547 +/- 108 mg L- 1 of total sugars. This value agrees with the predicted value (455 +/- 41 mg L- 1) by the statistical model. Through the SCB pretreated with Lac(mix) fermentation, 96.1% more H-2 and 22.5% more organic acids were observed compared to SCB without pretreatment. Therefore, laccases improve delignification, maximizing biomass fermentation for biofuel production. (AU) | |
| FAPESP's process: | 18/26470-7 - Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels |
| Grantee: | Camila Abreu Borges da Silva Rabelo |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/15455-4 - Contribution to integrated studies for leaf-cutting ants control: search for new insecticides and fungicides - nanoencapsulation |
| Grantee: | Hugo César Ramos de Jesus |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/06297-9 - Production and characterization of chimeric proteins containing chitinase catalytic domain and modules for chitin binding |
| Grantee: | Dulce Helena Ferreira de Souza |
| Support Opportunities: | Regular Research Grants |