Synergic recombinant enzyme association to optimize xylo-oligosaccharides production from agricultural waste

Author(s)

Martins, Manoela ; Avila, Patricia Felix ; Paim de Andrade, Cristiane Conte ; Goldbeck, Rosana

• Document type: Journal article
• Source: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 28, p. 8-pg., 2020-09-01.

Abstract

Xylo-oligosaccharides (XOS) are one of the classes of prebiotics obtained from lignocellulosic biomass, by hydrolysis of hemicellulose, abundant in agroindustrial residues. Several endoxylanases were studied in order to provide better conversion of the biomass to XOS. The resulting product presents multi-benefits, favoring the intestinal microbiota and maintaining the viability of probiotics in food. XOS production from sugarcane straw by action of thermostable recombinant endoxylanase of Cryptococcus fiavescens, expressed in Pichia pastoris GS115 using vector pGAPZ alpha A, in synergic association with alpha-L-arabinofuranosidase (GH 51) (Megazyme (R)) was optimized using a central composite rotatable design (CCRD), enabling 72.56% of xylan conversion. The main products xylobiose and xylotriose are short chain oligosaccharides preferred by probiotics. Hydrolysis of xylan from sugarcane straw using endoxylanase and alpha-L-arabinofuranosidase has great potential for XOS production with high prebiotic activity, representing a possible sustainable way to reduce enzymatic and raw material costs. (AU)

• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/14223-5 - Obtaining symbiotic microcapsules of xylo-oligosaccharides from sugarcane straw
• Grantee: Manoela Martins
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
• Grantee: Rosana Goldbeck Coelho
• Support Opportunities: Regular Research Grants