Bitter substances recovery from hot trub: A study of polymeric membranes performance in a sequential mode with fouling investigation

Author(s)

Silva, Klycia Fidelis Cerqueira e ; Rabelo, Renata Santos ; Feltre, Gabriela ; Hubinger, Miriam

• Document type: Journal article
• Source: Separation and Purification Technology; v. 303, p. 14-pg., 2022-10-08.

Abstract

Crossflow membrane technologies were used to clarify and concentrate the raw extract of bitter substances from hot trub, a brewing by-product, seeking to produce an alternative route for hop products via a study performance and fouling investigation. Microfiltration was performed in concentration (MF/CO) and diafiltration (MF/DF) operation modes, feeding with extract composed of lupulone/analogs (similar to 6 mg/g), iso-alpha-acid (similar to 4 mg/g), and humulones/analogs (similar to 2 mg/g). The MF/CO had similar to 69 % bitter substances permeation, while the MF/DF exhibited an increase of about 22 % in relation to MF/CO only (achieving 81-92 % the bitter substances permeation), being colupulone and n-+adlupulone presenting minor retention (<16 %). Adding the diafiltration step had not modified the profile of permeate fluxes curves, which was close to the exhibited by MF/CO. Concerning the sequential nanofiltration approach, six commercial membranes with different thin-film features and nominal molecular weight cut-offs ranging from 1000 to 150 g/mol (NP010 >= UA60 > NP030 > XN45 > NF9 > HL) were tested, operating with a clarified extract from MF/DF. XN45 and HL membranes presented higher bitter substance retention with less flux decay (similar to 42 %) than others. Membrane surfaces were altered after filtrations in relation to chemical profiles, morphology, and hydrophobicity, indicating a foulant layer formation. Different compositions and morphology of foulant layer were observed, being proteins the principal fouling agent for microfiltration while bitter substances were the main agent for nanofiltrations. (AU)

• FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 11/06083-0 - Emulsification by microchannels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/06863-7 - Use of protein and polysacharides to obtain structured oils:fat replacement and curcumin delivery
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/20466-8 - Overcoming barriers in the application of oleoresins: Stability and Digestibility of Co-encapsulated Systems with Application in Food Products
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/27354-3 - Architecture of colloidal delivery systems: what is the role of structure on the digestibility?
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Research Projects - Thematic Grants