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Different virulence genetic context of multidrug-resistant CTX-M- and KPC-producing Klebsiella pneumoniae isolated from cerebrospinal fluid

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Author(s):
Ballaben, Anelise Stella ; Galetti, Renata ; Ferreira, Joseane Cristina ; Paziani, Mario Henrique ; Kress, Marcia Regina von Zeska ; Garcia, Doroti de Oliveira ; da Silva, Paulo ; Doi, Yohei ; Darini, Ana Lucia Costa ; Andrade, Leonardo Neves
Total Authors: 10
Document type: Journal article
Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 104, n. 3, p. 5-pg., 2022-08-19.
Abstract

Information regarding resistance and virulence traits of meningitis-causing enterobacteria in hospital environment remains scarce. The aim of this study was to characterize virulence and acquired resistance genes of carbapenem-resistant and/or 3rd to 4th generation cephalosporin-resistant Klebsiella pneumoniae isolated from the cerebrospinal fluid of inpatients. Antimicrobial susceptibility testing was performed by disk diffusion. The string test was performed to identify hypermucoviscous phenotype. Galleria mellonella infection model was used to evaluate the virulence profile of the isolates. Screening for virulence determinants and acquired resistance genes were performed by PCR. The blaCTX-M and/or blaKPC and/or rmtG were detected in all the isolates. Genetic virulence determinants, including mrkD, entB, iroD, fecIRA, uge, wabG, fimH, ureA, ybtS, and clb were detected in the majority of multidrug-resistant K. pneumoniae isolates. One isolate presented hypermucoviscous phenotype, and several isolates showed enhanced virulence in G. mellonella infection model. The combination of the virulence genes found here seems to support not only the known virulence genetic context among nosocomial infections-causing K. pneumoniae but also the role that clb and ybtS may play in K. pneumoniae virulence. (C) 2022 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 15/11728-0 - Complete sequencing of plasmids carrying antibiotic resistance genes in bacteria of interest and genome analysis of Pseudomonas aeruginosa-producing SPM-1 and KPC-2 lines by large-scale sequencing
Grantee:Renata Galetti
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
Grantee:Ana Lúcia da Costa Darini
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/23484-9 - Molecular study of gram-negative beta-lactamase producing bacteria isolated from patients with suspected meningitis in the Ribeirão Preto region
Grantee:Anelise Stella Ballaben
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)