Nanographene laser-pyrolyzed paper electrodes for the impedimetric detection of D-glucose via a molecularly imprinted polymer

Author(s)

Bossard, Bruna ; Grothe, Renata Antunes ; Martins, Alisson Bezerra ; Lobato, Alnilan ; Tasic, Nikola ; Paixao, Thiago R. L. C. ; Goncalves, Luis Moreira

• Document type: Journal article
• Source: MONATSHEFTE FUR CHEMIE; v. 153, n. 12, p. 7-pg., 2022-10-14.

Abstract

In this proof-of-concept study, we used laser-pyrolyzed paper as the supporting electrodes for the swift, impedimetric, and enzymeless detection of D-glucose by the means of molecularly imprinted polymer. Molecular imprinting of monomer 3-amino-4-hydroxybenzoic acid was performed by electrochemical polymerization in the presence of D-glucoseas a template molecule, followed by the template removal process via cyclic voltammetry in PBS (pH = 7.4). The analytical capacitance signal was extracted from the raw electrochemical impedance spectra recorded after only 60 s of incubation with the analyte. The electrodes were characterized by Raman spectroscopy and scanning electron microscopy to disclose their structural and morphological properties, revealing the presence of nanographene of high porosity. The analytical performance was investigated in the clinically relevant range of 0-30 mmol dm(-3), which demonstrated good linearity (r(2) = 0.97), decent limit of detection (1.77 mmol dm(-3)), an excellent selectivity (a = 1.38-3.73), and good reproducibility (5.0%). (AU)

• FAPESP's process: 18/14425-7 - A new synergetic path for gas-diffusion microextraction (GDME) making use of molecular imprinted polymers (MIPs)
• Grantee: Luís Francisco Moreira Gonçalves
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/10522-5 - Development of alternative methods for fabrication of electrochemical paper-based sensors
• Grantee: Thiago Regis Longo Cesar da Paixão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/13922-7 - Development of a point-of-care biosensor for the swift determination of D-Dimers
• Grantee: Nikola Tasic
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/11214-8 - Application of capillary electrophoresis for the determination of aldehydes after extraction by GDME-MIPs
• Grantee: Alnilan Cristina Barros Lobato
• Support Opportunities: Scholarships in Brazil - Master