Electrochemical Biosensor for SARS-CoV-2 cDNA Detection Using AuPs-Modified 3D-Printed Graphene Electrodes

Author(s)

Silva, Luiz R. G. ; Stefano, Jessica S. ; Orzari, Luiz O. ; Brazaca, Lais C. ; Carrilho, Emanuel ; Marcolino-Junior, Luiz H. ; Bergamini, Marcio F. ; Munoz, Rodrigo A. A. ; Janegitz, Bruno C.

• Document type: Journal article
• Source: BIOSENSORS-BASEL; v. 12, n. 8, p. 20-pg., 2022-08-01.

Abstract

A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electrode were involved in a complexation reaction with creatinine, resulting in a decrease in the analytical response, and thus providing a fast and simple electroanalytical device. Physicochemical characterizations were performed by SEM, EIS, FTIR, and cyclic voltammetry. Square wave voltammetry was employed for the creatinine detection, and the sensor presented a linear response with a detection limit of 0.016 mmol L-1. Finally, a biosensor for the detection of SARS-CoV-2 was developed based on the immobilization of a capture sequence of the viral cDNA upon the Au-modified 3D-printed electrode. The concentration, immobilization time, and hybridization time were evaluated in presence of the DNA target, resulting in a biosensor with rapid and low-cost analysis, capable of sensing the cDNA of the virus with a good limit of detection (0.30 mu mol L-1), and high sensitivity (0.583 mu A mu mol(-1 )L). Reproducible results were obtained (RSD = 1.14%, n = 3), attesting to the potentiality of 3D-printed platforms for the production of biosensors. (AU)

• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 22/06145-0 - Obtention of improved electrochemical sensors by modifying 3D printed electrodes with biofilms obtained from beeswax and carbon nanomaterials for the detection of harmful compounds in honey
• Grantee: Jéssica Santos Stefano
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/19750-3 - Exploring C4D detection for the development of innovative and low-cost microfluidic biosensors
• Grantee: Laís Canniatti Brazaca
• Support Opportunities: Scholarships in Brazil - Post-Doctoral