Pressurized liquid extraction coupled in-line with SPE and on-line with HPLC (PLE-SPExHPLC) for the recovery and purification of anthocyanins from SC-CO2 semi-defatted Acai (Euterpe oleracea)

Author(s)

Maciel-Silva, Francisco W. ; Vigano, Juliane ; Castro, Luiz E. N. ; Sganzerla, William Gustavo ; Buller, Luz Selene ; Martinez, Julian ; Rostagno, Mauricio A. ; Forster-Carneiro, Tania

• Document type: Journal article
• Source: Food Research International; v. 160, p. 11-pg., 2022-07-26.

Abstract

This study aimed to extract anthocyanins from dried and semi-defatted acai pulp using green technologies based on the coupling of pressurized liquid extraction (PLE) with in-line purification through solid-phase extraction (SPE) and on-line analysis by high-performance liquid chromatography (HPLC). Critical parameters that affect the extraction efficiency and purification were investigated and optimized by response surface methodology (RSM). PLE was performed with acidified water at different pH (2.0, 4.5, and 7.0) and temperatures (40, 80, and 120 degrees C) at 15 MPa, 2 mL/min, and solvent-to-feed mass ratio equal to 40. SPE was optimized in a column packed with the adsorbent PoraPakTM Rxn. Different ethanol concentrations (50, 75, and 100 %) and temperatures (30, 40, and 50 degrees C) were evaluated for the anthocyanin's elution. The optimal conditions of the two experimental designs were determined by the RSM, firstly for PLE: 71 degrees C and pH 2; then using this PLE condition, the opti-mization of the SPE was obtained: 30 degrees C and 50 % ethanol. The developed PLE method provided similar anthocyanin yield to other techniques, and the coupling with SPE in-line produced an extract 5-fold more concentrated than PLE alone. Therefore, the system (PLE-SPE x HPLC-PDA) proved to be a powerful tool for monitoring the extraction process in real-time. (AU)

• FAPESP's process: 20/10323-5 - Life cycle and technical-financial assessment of the integration of anaerobic digestion and hydrothermal technologies for waste recovery
• Grantee: Luz Selene Buller
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
• Grantee: Juliane Viganó
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/14938-4 - Waste2energy: integration of supercritical technologies and anaerobic digestion through an automated system for the production of biofuel precursors
• Grantee: Tânia Forster Carneiro
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 18/05999-0 - Waste2energy: integration of the supercritical and anaerobic digestion technologies for the management of water and food waste
• Grantee: Tânia Forster Carneiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/26925-7 - Development of an automated system for biofuel precursor production using sub-critical technology
• Grantee: William Gustavo Sganzerla
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2