Latex-collagen membrane: an alternative treatment for tibial bone defects

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de Paiva, Marcela Britto ; Brasil, Giovana Sant'Ana Pegorin ; Chagas, Ana Laura Destro ; Macedo, Ana Paula ; Ramos, Junia ; Issa, Joao Paulo Mardegan ; Gangrade, Ankit ; Floriano, Juliana Ferreira ; Caetano, Guilherme Ferreira ; Li, Bingbing ; Farhadi, Neda ; Mandal, Kalpana ; Dokmeci, Mehmet Remzi ; Jucaud, Vadim ; Herculano, Rondinelli Donizetti ; Shimano, Antonio Carlos

• Document type: Journal article
• Source: Journal of Materials Science; v. N/A, p. 23-pg., 2022-12-08.

Abstract

Due to the difficulty of consolidation, the risk of fractures, and the high cost of therapy, the orthopedic field views the restoration of bony defects or post-traumatic diseases as a significant issue. In recent years, efforts have been made toward guided bone regeneration as a viable solution. In this study, natural rubber latex (NRL) and latex-collagen (NRL-COL) membranes were developed and characterized, and their effects on bone regeneration in rat tibial lesions were assessed. According to the physicochemical findings, the hydrolyzed COL was effectively incorporated into the NRL membrane to produce a more robust membrane (348% increase in stress) and showed good biocompatibility for human dermal fibroblast cells and in hemolytic activity assay. According to bone analysis using computed microtomography, a substantial difference in bone volume (BV) was found among NRL, control (CONT), and NRL-COL. NRL-COL exhibited the greatest value for the bone volume/total volume ratio (BV/TV). The presence of NRL and COL was significant for the analysis of BV and BV/TV, respectively. All the experimental groups demonstrated a higher bone quality and fracture resistance, as shown by the mechanical properties of the bone. In the COL group, both neoformation of bone and total collagen levels were greater, indicating that treated defects had been closed and mature bone cells were present. Due to its angiogenic properties and the low cost of NRL membrane production, the NRL-COL membrane could be a good substitute for upcoming bone regeneration applications. (AU)

• FAPESP's process: 14/17526-8 - Development of a novel system employing natural rubber latex as releasing of vegetable extracts incorporated with nanoparticles: physical chemical and biological characterization
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/17411-8 - Evaluation of drugs delivery systems using natural rubber latex membranes as carrier
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/19603-8 - Development of a novel patch containing latex and barbatimam e fractions and study of signaling pathways in cutaneous wound repair
• Grantee: Rondinelli Donizetti Herculano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/20051-0 - Regeneration of bone defects using different therapeutic measures
• Grantee: Antônio Carlos Shimano
• Support Opportunities: Regular Research Grants