A reliable validated high-performance liquid chromatography-photodiode array detection method for quantification of terpenes in Copaifera pubiflora, Copaifera trapezifolia, and Copaifera langsdorffii oleoresins

Author(s)

Carneiro, Luiza J. ; Bastos, Jairo K. ; Veneziani, Rodrigo C. S. ; Santos, Mario F. C. ; Ambrosio, Sergio R.

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. N/A, p. 6-pg., 2022-08-22.

Abstract

(The) Copaifera oleoresins are widely used in folk medicine to treat various diseases. The goal of this study was to develop a validated reverse-phase high-performance liquid chromatography method with photodiode array detection (RP-HPLC-PDA) to quantify eight terpenes: ent-hardwickiic acid, ent-copalic acid, ent-7 alpha-acetoxy hardwickiic acid, ent-16-hydroxy-3,13-clerodadiene-15,18-dioic acid, ent-5,13-labdadiene-15-oic acid, junenol, ent-kaurenoic acid, and 13E-ent-labda-7,13-dien-15-oic acid in the oleoresins of Copaifera pubiflora L. (OCP), Copaifera trapezifolia L. (OCT) and Copaifera langsdorffii L. (OCL). The linearity of the method was confirmed in the range of 20.00-500 mu g.mL(-1) (r(2) > 0.999). The limit of quantification was between 1,05 and 16.89 mu g.mL(-1). Precision and accuracy ranges were found to be %RSD <0.2 and 96% to 110%, respectively. Based on the obtained results, the developed analytical method is rapid, precise, accurate, and sensitive for quantifying these terpenes in Copaifera's oleoresins. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/25010-2 - Isolation and identification of green and brown propolis constituents.
• Grantee: Mário Ferreira Conceição Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral