Synergism for xylo-oligosaccharides, p-coumaric and ferulic acid production, and thermostability modulation of GH 62 alpha-L-arabinofuranosidase

Author(s)

Martins, Manoela ; Tramontina, Robson ; Squina, Fabio Marcio ; Dinamarco, Taisa Magnani ; Goldbeck, Rosana

• Document type: Journal article
• Source: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 44, p. 11-pg., 2022-08-26.

Abstract

GH 62 alpha-L-arabinofuranosidases are a group of enzymes that preferably hydrolyze L-arabinose residues of arabinoxylans. Albeit the least studied, these enzymes have gained attention because they exert synergistic action with other xylanolytic enzymes for arabinoxylan depolymerization. This is the first study on the thermodynamic and kinetic parameters, structural prediction, and metal ion effect on the thermostability of a GH 62 arabinofuranosidase subfamily 1. The optimum enzymatic conditions are pH 4.8 and 42 C, and the presence of Ca2+ and Mn2+ ions enhanced the thermostability and activity. The synergism of this arabinofuranosidase with a GH 10 endox-ylanase increased the production of xylo-oligosaccharides 2.3 times with additional coproduction of 0.82 mM of ferulic acid and 0.09 mM of p-coumaric acid released from corn hull arabinoxylan (4% w/v). These results are relevant to developing an enzymatic formulation that is economically feasible for industrial applications, especially for prebiotic and antioxidant compounds produc-tion in the food and pharmaceutical sectors from sources rich in arabinoxylan, especially agro-industrial wastes of grains husks and peels. (AU)

• FAPESP's process: 20/02871-2 - Bifunctional chimeric protein of endoxylanase/arabinofuranosidase for agro-industrial wastes hydrolysis and xylooligosaccharides production
• Grantee: Manoela Martins
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
• Grantee: Rosana Goldbeck Coelho
• Support Opportunities: Regular Research Grants