Molecular characterization and sequecing analysis of SARS-CoV-2 genome in Minas Gerais, Brazil

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Ferreira, Giulia Magalhaes ; Claro, Ingra Morales ; Grosche, Victoria Riquena ; Candido, Darlan ; Jose, Diego Pandelo ; Rocha, Esmenia Coelho ; Coletti, Thais de Moura ; Manuli, Erika Regina ; Gaburo Jr, Nelson ; Faria, Nuno Rodrigues ; Sabino, Ester Cerdeira ; de Jesus, Jaqueline Goes ; Jardim, Ana Carolina Gomes

• Document type: Journal article
• Source: BIOLOGICALS; v. 80, p. 10-pg., 2022-10-01.

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first identified in Wuhan, China, is the causative agent of the coronavirus disease 2019 (COVID-19). Since its first notification in Sa similar to o Paulo state (SP) on 26th February 2020, more than 22,300,000 cases and 619,000 deaths were reported in Brazil. In early pandemic, SARS-CoV-2 spread locally, however, over time, this virus was disseminated to other regions of the country. Herein, we performed genomic sequencing and phylogenetic analysis of SARS-CoV-2 using 20 clinical samples of COVID-19 confirmed cases from 9 cities of Minas Gerais state (MG), in order to evaluate the molecular properties of circulating viral strains in this locality from March to May 2020. Our analyses demonstrated the circulation of B.1 lineage isolates in the investigated locations and nucleotide substitutions were observed into the genomic regions related to important viral structures. Additionally, sequences generated in this study clustered with isolates from SP, suggesting a dissemination route between these two states. Alternatively, monophyletic groups of sequences from MG and other states or country were observed, indicating independent events of virus introduction. These results reinforce the need of genomic surveillance for understand the ongoing spread of emerging viral pathogens. (AU)

• FAPESP's process: 18/17176-8 - Real-time, rapid detection and sequencing of arboviruses in Brazil: Zika, Dengue, Chikungunya and Yellow Fever
• Grantee: Ingra Morales Claro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/14389-0 - Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE)
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/12000-1 - Modeling of the spatiotemporal dispersion of emergent arboviruses using heterogeneous data sources in the State of São Paulo
• Grantee: Jaqueline Goes de Jesus
• Support Opportunities: Scholarships in Brazil - Post-Doctoral