Biological responses to imazapic and methyl parathion pesticides in bioinspired lipid membranes and Tilapia fish

Author(s)

Rubira, Rafael J. G. ; Batista, Victor R. G. ; Correia, Rafael R. ; Pazin, Wallance M. ; Maximino, Mateus D. ; Ruiz, Gilia C. M. ; Teixeira, Giovana R. ; Job, Aldo E.

• Document type: Journal article
• Source: JOURNAL OF HAZARDOUS MATERIALS; v. 458, p. 12-pg., 2023-06-28.

Abstract

Pesticide misuse has well-documented detrimental effects on ecosystems, with Nile tilapia (Oreochromis niloticus) being particularly vulnerable. The current study focuses on the impact of widely used sugarcane crop pesticides, Imazapic (IMZ) and Methyl Parathion (MP), on tilapia gill tissues and their lipid membranes. This investigation was motivated by the specific role of the lipid membrane in transport regulation. Bioinspired cell membrane models, including Langmuir monolayers and liposomes (LUVs and GUVs), were utilized to explore the interaction of IMZ and MP. The results revealed electrostatic interactions between IMZ and MP and the polar head groups of lipids, inducing morphological alterations in the lipid bilayer. Tilapia gill tissue exposed to the pesticides exhibited hypertrophic increases in primary and secondary lamellae, total lamellar fusion, vasodilation, and lifting of the secondary lamellar epithelium. These alterations can lead to compromised oxygen absorption by fish and subsequent mortality. This study not only highlights the harmful effects of the pesticides IMZ and MP, but also emphasizes the crucial role of water quality in ecosystem well-being, even at minimal pesticide concentrations. Understanding these impacts can better inform management practices to safeguard aquatic organisms and preserve ecosystem health in pesticide-affected environments. (AU)

• FAPESP's process: 20/05423-0 - Detection of pesticides (cocktails) used in sugarcane crops and their effect on biological systems
• Grantee: Rafael Jesus Gonçalves Rubira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/15324-0 - Effects of high intensity interval physical training (HIIT) on the histological, biochemical and molecular parameters of the urinary bladder of spontaneously hypertensive rats (SHR)
• Grantee: Victor Rogério Garcia Batista
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/16310-2 - Development of biotechnology applied to prostatic carcinomatosis with the use of Docetaxel chemotherapy care nanoparticles associated with aerobic physical training
• Grantee: Giovana Rampazzo Teixeira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/15185-0 - Lipid nanoparticles for encapsulation and carrier of natural products: methods of preparation, comparison and optimization
• Grantee: Gilia Cristine Marques Ruiz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/14514-2 - Analysis of cancer cachexia associated with the treatment of drug-carrying nanoparticles of docetaxel and methotrexate in sprague-dawley rats submitted to aerobic physical training
• Grantee: Rafael Ribeiro Correia
• Support Opportunities: Scholarships in Brazil - Master