The Versatility of Two-Dimensional Liquid Chromatography

Author(s)

Cardoso, Carmen L. ; de Moraes, Marcela C. ; Cass, Quezia B.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. N/A, p. 16-pg., 2023-07-20.

Abstract

This review deals with two-dimensional liquid chromatography (2D-LC) separations encompassing target heart-cut (LC-LC), multiple heart-cut (mLC-LC), non-targeted comprehensive (LC x LC), and selective comprehensive (sLC x LC) analysis. It presents an overview of basic concepts and emphasizes the versatility of the applications gained by going from one-(1D) to two-dimensional (2D) separations. This review also discusses target analysis of achiral and chiral drugs for different applications and the use of 2D-LC in zonal bioaffinity chromatography. Advances in instrumental and column technologies have widened the application of LC x LC and sLC x LC separations, and we will discuss some of them. (AU)

• FAPESP's process: 22/00432-7 - Integrated platforms for screening enzyme ligands: from development to application
• Grantee: Carmen Lúcia Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/05363-0 - Enzyme ligand screening: an on-flow approach via LC-MS/MS
• Grantee: Carmen Lúcia Cardoso
• Support Opportunities: Regular Research Grants