Additive Manufacturing Sensor for Stress Biomarker Detection

Author(s)

da Silva, Vinicius A. O. P. ; Stefano, Jessica S. ; Kalinke, Cristiane ; Bonacin, Juliano. A. A. ; Janegitz, Bruno. C. C.

• Document type: Journal article
• Source: CHEMOSENSORS; v. 11, n. 5, p. 14-pg., 2023-05-20.

Abstract

This work presents a new additive manufacturing electrochemical device with conductive graphene and polylactic acid (PLA) filament and its application for epinephrine sensing. A three-electrode configuration based on a screen-printed electrode architecture and an easy-to-connect connector was designed. The sensor surface was chemically treated with dimethylformamide (DMF) to remove the insulating thermoplastic and expose the graphene binding groups. The scanning electron microscopy (SEM) results showed that the surface PLA was removed and the graphene nanofibers exposed, which corroborated the X-ray diffraction spectra (XRD). As a proof of concept, the G-PLA electrode was applied for the determination of epinephrine in human blood samples by square wave voltammetry with a linear range from 4.0 to 100 mu mol L-1 and a limit of detection of 0.2 mu mol L-1. Based on the results obtained and sensor application, 3D-printed G-PLA proved an excellent choice for epinephrine sensing purposes. (AU)

• FAPESP's process: 22/06145-0 - Obtention of improved electrochemical sensors by modifying 3D printed electrodes with biofilms obtained from beeswax and carbon nanomaterials for the detection of harmful compounds in honey
• Grantee: Jéssica Santos Stefano
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 21/03179-8 - New architectures of 3D printed sensors for detection of biomarkers and hydrogen peroxide
• Grantee: Vinicius Aparecido Oliani Pedro da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/07989-4 - Additive-manufactured (3D printed) electrochemical devices for the diagnosis of viral diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor