Ethanolamine phospholipids at the air-water interface as cell membranes models of microorganisms to study the nanotoxicology of sakuranetin

Author(s)

Pires, Guilherme Henrique da Cruz Ramos ; Barbosa, Henrique ; Almeida, Roberto Baptista Pereira ; Lago, Joao Henrique Ghilardi ; Caseli, Luciano

• Document type: Journal article
• Source: Thin Solid Films; v. 770, p. 8-pg., 2023-03-01.

Abstract

Sakuranetin, a natural flavonoid isolated as the main compound from roots of the Brazilian plant Baccharis retusa (Asteraceae), was incorporated in lipid membranes as Langmuir monolayers. For that, the mammalian lipid dipalmitoyl phosphatidylethanolamine (DPPE) was chosen. Sakuranetin condensed the monolayers at high surface pressures, decreased the surface compression modulus and reduced the molecular order of the acyl chains (reduction of all-trans/gauche conformers ratio), and increased the heterogeneity of the interface, forming ag-gregates. These combined results lead to the conclusion that this lipophilic compound may interact with the lipidic layers, preferentially at the lipid-air interface, to minimize the free energy, and reaches such conformation disturbing the thermodynamic, structural, mechanical, rheological, and morphological properties of the well-packed DPPE monolayer. (AU)

• FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/18125-0 - Computational study of materials with application in energy storage
• Grantee: Leonardo José Amaral de Siqueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/02789-7 - Search for bioactive metabolites with antiparasitic action in plant species from Atlantic Forest and Cerrado regions - a chemical, phenotypical, and metabolomic approach
• Grantee: João Henrique Ghilardi Lago
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 22/03736-7 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants