Caffeine Release from Magneto-Responsive Hydrogels Controlled by External Magnetic Field and Calcium Ions and Its Effect on the Viability of Neuronal Cells

Author(s)

Frachini, Emilli C. G. ; Selva, Jessica S. G. ; Falcoswki, Paula C. ; Silva, Jean B. ; Cornejo, Daniel R. ; Bertotti, Mauro ; Ulrich, Henning ; Petri, Denise F. S.

• Document type: Journal article
• Source: POLYMERS; v. 15, n. 7, p. 22-pg., 2023-04-01.

Abstract

Caffeine (CAF) is a psychostimulant present in many beverages and with rapid bioab-sorption. For this reason, matrices that effectuate the sustained release of a low amount of CAF would help reduce the intake frequency and side effects caused by high doses of this stimulant. Thus, in this study, CAF was loaded into magnetic gelatin/alginate (Gel/Alg/MNP) hydrogels at 18.5 mg/ghydrogel. The in vitro release of CAF was evaluated in the absence and presence of an external magnetic field (EMF) and Ca2+. In all cases, the presence of Ca2+ (0.002 M) retarded the release of CAF due to favorable interactions between them. Remarkably, the release of CAF from Gel/Alg/MNP in PBS/CaCl2 (0.002 M) at 37 degrees C under an EMF was more sustained due to synergic effects. In PBS/CaCl2 (0.002 M) and at 37 degrees C, the amounts of CAF released after 45 min from Gel/Alg and Gel/Alg/MNP/EMF were 8.3 +/- 0.2 mg/ghydrogel and 6.1 +/- 0.8 mg/g(hydrogel), respectively. The concentration of CAF released from Gel/Alg and Gel/Alg/MNP hydrogels amounted to similar to 0.35 mM, thereby promoting an increase in cell viability for 48 h. Gel/Alg and Gel/Alg/MNP hydrogels can be applied as reservoirs to release CAF at suitable concentrations, thus forestalling possible side effects and improving the viability of SH-SY5Y cells. (AU)

• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/07366-4 - Purine and kinin receptors as targets of study and therapeutic interventions in neurological diseases
• Grantee: Alexander Henning Ulrich
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants