| Full text | |
| Author(s): |
Frachini, Emilli C. G.
;
Selva, Jessica S. G.
;
Falcoswki, Paula C.
;
Silva, Jean B.
;
Cornejo, Daniel R.
;
Bertotti, Mauro
;
Ulrich, Henning
;
Petri, Denise F. S.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | POLYMERS; v. 15, n. 7, p. 22-pg., 2023-04-01. |
| Abstract | |
Caffeine (CAF) is a psychostimulant present in many beverages and with rapid bioab-sorption. For this reason, matrices that effectuate the sustained release of a low amount of CAF would help reduce the intake frequency and side effects caused by high doses of this stimulant. Thus, in this study, CAF was loaded into magnetic gelatin/alginate (Gel/Alg/MNP) hydrogels at 18.5 mg/ghydrogel. The in vitro release of CAF was evaluated in the absence and presence of an external magnetic field (EMF) and Ca2+. In all cases, the presence of Ca2+ (0.002 M) retarded the release of CAF due to favorable interactions between them. Remarkably, the release of CAF from Gel/Alg/MNP in PBS/CaCl2 (0.002 M) at 37 degrees C under an EMF was more sustained due to synergic effects. In PBS/CaCl2 (0.002 M) and at 37 degrees C, the amounts of CAF released after 45 min from Gel/Alg and Gel/Alg/MNP/EMF were 8.3 +/- 0.2 mg/ghydrogel and 6.1 +/- 0.8 mg/g(hydrogel), respectively. The concentration of CAF released from Gel/Alg and Gel/Alg/MNP hydrogels amounted to similar to 0.35 mM, thereby promoting an increase in cell viability for 48 h. Gel/Alg and Gel/Alg/MNP hydrogels can be applied as reservoirs to release CAF at suitable concentrations, thus forestalling possible side effects and improving the viability of SH-SY5Y cells. (AU) | |
| FAPESP's process: | 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine |
| Grantee: | Luiz Henrique Catalani |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/07366-4 - Purine and kinin receptors as targets of study and therapeutic interventions in neurological diseases |
| Grantee: | Alexander Henning Ulrich |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications |
| Grantee: | Mauro Bertotti |
| Support Opportunities: | Research Projects - Thematic Grants |