Efficiency of Different Solvents on the Extraction of Bioactive Compounds from the Amazonian Fruit Caryocar villosum and the Effect on its Antioxidant and Colour Properties

Author(s)

Chiste, Renan Campos ; Benassi, Marta de Toledo ; Mercadante, Adriana Zerlotti

• Document type: Journal article
• Source: Phytochemical Analysis; v. 25, n. 4, p. 9-pg., 2014-07-01.

Abstract

Introduction - Caryocar villosum has been reported as a source of bioactive compounds that can be used as a potential product against oxidative damage in foods or biological systems. Objective - To obtain extracts from fruit pulps of C. villosum with high levels of bioactive compounds that have both antioxidant and colour properties. Method - The contents of bioactive compounds (total phenolic compounds, flavonoids, tannins, carotenoids and tocopherols), the colour parameters, the scavenging capacity against peroxyl radicals (ROO center dot) and the quenching activity against singlet oxygen (O-1(2)) were determined. All data were used for extract classification by applying principal components analysis and hierarchical cluster analysis. Results - The water and ethanol: water (1:1, v/v) extracts presented the highest levels of total phenolic compounds (9.2 and 6.3mg gallic acid equivalent/g extract, respectively), total flavonoids (3.8 and 2.5mg catechin equivalent/g extract, respectively) and total tannins (7.6 and 2.4 mg tannic acid equivalent/g extract, respectively). The ethanol: water (1: 1, v/v) extract also showed the highest scavenging capacity against ROO center dot (0.3 mmol trolox equivalent/g extract) and the highest protection against O-1(2) (12.5%). On the other hand, the ethanol extracts, which were the most vivid and yellow colour (C-ab* = 13.7 and b* = 13.3), presented the highest level of total carotenoids (0.1 mg/g), but low scavenging capacity against ROO center dot (0.01 mmol trolox equivalent/g extract). Conclusion - Based on these results and depending on the applicability, the ethanol: water, water and ethanol are the most promising solvents to obtain C. villosum extracts with high contents of bioactive compounds, ROO center dot scavenging capacity and protection against O-1(2). Copyright (C) 2013 John Wiley & Sons, Ltd. (AU)

• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC