Surface plasmon resonance biosensor for enzymatic detection of small analytes

Author(s)

Miyazaki, Celina Massumi ; Shimizu, Flavio Makoto ; Mejia-Salazar, J. R. ; Oliveira, Osvaldo N., Jr. ; Ferreira, Marystela

• Document type: Journal article
• Source: Nanotechnology; v. 28, n. 14, p. 6-pg., 2017-04-07.

Abstract

Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. In this paper, we report on a SPR biosensing platform suitable to detect small molecules by making use of the mediator-type enzyme microperoxidase-11 (MP11) in layer-by-layer films. By depositing a top layer of glucose oxidase or uricase, we were able to detect glucose or uric acid with limits of detection of 3.4 and 0.27 mu mol l(-1), respectively. Measurable SPR signals could be achieved because of the changes in polarizability of MP11, as it is oxidized upon interaction with the analyte. Confirmation of this hypothesis was obtained with finite difference time domain simulations, which also allowed us to discard the possible effects from film roughness changes observed in atomic force microscopy images. The main advantage of this mediator-type enzyme approach is in the simplicity of the experimental method that does not require an external potential, unlike similar approaches for SPR biosensing of small molecules. The detection limits reported here were achieved without optimizing the film architecture, and therefore the performance can in principle be further enhanced, while the proposed SPR platform may be extended to any system where hydrogen peroxide is generated in enzymatic reactions. (AU)

• FAPESP's process: 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue
• Grantee: Flavio Makoto Shimizu
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
• Grantee: Celina Massumi Miyazaki
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/12311-9 - Transversal magneto-optical Kerr effect for the design and development of biosensors
• Grantee: Jorge Ricardo Mejía Salazar
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/17519-1 - Immobilization of metal nanoparticules in ultrathin films aiming biosensing
• Grantee: Marystela Ferreira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants