Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide

Author(s)

Araujo, Claudineia A. S. ; Dias, Luciana P. ; Ferreira, Paulo C. ; Mittmann, Josane ; Pupin, Breno ; Brancini, Guilherme T. P. ; Braga, Gilberto U. L. ; Rangel, Drauzio E. N.

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 122, n. 6, p. 8-pg., 2018-06-01.

Abstract

Survival of entomopathogenic fungi under solar ultraviolet (UV) radiation is paramount to the success of biological control of insect pests and disease vectors. The mutagenic compound 4-nitroquinoline 1-oxide (4-NQO) is often used to mimic the biological effects of UV radiation on organisms. Therefore, we asked whether tolerance to 4-NQO could predict tolerance to UV radiation in thirty isolates of entomopathogenic fungi and one isolate of a xerophilic fungus. A dendrogram obtained from cluster analyses based on the 50 and 90 % inhibitory concentrations (IC50 and IC90, respectively) divided the fungal isolates into six clusters numbered consecutively based on their tolerance to 4-NQO. Cluster 6 contained species with highest tolerance to 4-NQO (IC50 > 4.7 mu M), including Mariannaea pruinosa, Lecanicillium aphanocladii, and Torrubiella homopterorum. Cluster 1 contained species least tolerant to 4-NQO (IC50 < 0.2 mu M), such as Metarhizium acridum (ARSEF 324), Tolypocladium geodes, and Metarhizium brunneum (ARSEF 7711). With few exceptions, the majority of Metarhizium species showed moderate to low tolerances (IC50 between 0.4 and 0.9 mu M) and were placed in cluster 2. Cluster 3 included species with moderate tolerance (IC50 between 1.0 and 1.2 M). In cluster 4 were species with moderate to high tolerance (IC50 between 1.3 and 1.6 mu M). Cluster 5 contained the species with high tolerance (IC50 between 1.9 and 4.0 mu M). The most UV tolerant isolate of M. acridum, ARSEF 324, was the least tolerant to 4-NQO. Also, L aphanocladii, which is very susceptible to UV radiation, showed high tolerance to 4-NQO. Our results indicate that tolerance to 4-NQO does not correlate with tolerance to UV radiation. Therefore this chemical compound is not a predictor of UV tolerance in entomopathogenic fungi. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
• Grantee: Drauzio Eduardo Naretto Rangel
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/25964-2 - Visible light during mycelial growth alters gene expression and improves stress tolerance in entomopathogenic fungi
• Grantee: Luciana Pereira Dias
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
• Grantee: Gilberto Úbida Leite Braga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
• Grantee: Drauzio Eduardo Naretto Rangel
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
• Grantee: Gilberto Úbida Leite Braga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/24305-0 - Evaluating the effects of blue and red light on the stress tolerance and the proteome of the entomopathogenic fungus Metarhizium acridum
• Grantee: Guilherme Thomaz Pereira Brancini
• Support Opportunities: Scholarships in Brazil - Doctorate