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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial diversity of periodontal and implant-related sites detected by the DNA Checkerboard method

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Author(s):
do Nascimento, C. [1, 2] ; Monesi, N. [3] ; Ito, I. Y. [3] ; Issa, J. P. M. [4] ; de Albuquerque Junior, R. F. [2]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Dent Ribeirao Preto, Dept Morphol & Physiol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES; v. 30, n. 12, p. 1607-1613, DEC 2011.
Web of Science Citations: 9
Abstract

The aim of this study was to compare the microbial composition of the subgingival biofilm from teeth and implant sulci in relation to contents originating from internal parts of the implant, abutment and implant prosthesis. Twenty subgingival biofilm samples from the mesial and distal aspects of each tooth/implant and 29 samples from the internal parts of titanium implants, abutments and implant prostheses were evaluated for the presence of 18 bacterial species using DNA Checkerboard and the differences between samples from teeth and implants were assessed with Pearson's correlation analysis. The periodontal and peri-implantar sulci presented significantly higher bacterial counts than the implant-related sites (p < 0.05 and p < 0.01, respectively). The highest counts were observed for Capnocytophaga gingivalis, Prevotella intermedia, P. nigrescens and P. micra. The correlation between the counts in the periodontal and peri-implantar sulci was r = 0.66 (p < 0.001). Weaker correlations between samples from the internal parts of the implants and periodontal sulcus (r = 0.49; p < 0.001) or peri-implant sulcus (r = 0.42; p < 0.001) were found. All 18 bacterial species were detected to be colonising the subgingival sulcus of teeth and implants, and implant components in the evaluated patients. Significant correlations between the microbiota were found, the strongest being between the periodontal and peri-implantar sulci. (AU)