| Full text | |
| Author(s): Show less - |
Pinzan, Camila Figueiredo
;
Valero, Clara
;
de Castro, Patricia Alves
;
da Silva, Jefferson Luiz
;
Earle, Kayleigh
;
Liu, Hong
;
Horta, Maria Augusta Crivelente
;
Kniemeyer, Olaf
;
Kruger, Thomas
;
Pschibul, Annica
;
Comert, Derya Nur
;
Heinekamp, Thorsten
;
Brakhage, Axel A.
;
Steenwyk, Jacob L.
;
Mead, Matthew E.
;
Hermsdorf, Nico
;
Filler, Scott G.
;
da Rosa-Garzon, Nathalia Gonsales
;
Delbaje, Endrews
;
Bromley, Michael J.
;
Cabral, Hamilton
;
Diehl, Camila
;
Angeli, Claudia B.
;
Palmisano, Giuseppe
;
Ibrahim, Ashraf S.
;
Rinker, David C.
;
Sauters, Thomas J. C.
;
Steffen, Karin
;
Gumilang, Adiyantara
;
Rokas, Antonis
;
Gago, Sara
;
dos Reis, Thaila F.
;
Goldman, Gustavo H.
Total Authors: 33
|
| Document type: | Journal article |
| Source: | NATURE MICROBIOLOGY; v. 9, n. 10, p. 30-pg., 2024-08-27. |
| Abstract | |
Aspergillus fumigatus causes aspergillosis and relies on asexual spores (conidia) for initiating host infection. There is scarce information about A. fumigatus proteins involved in fungal evasion and host immunity modulation. Here we analysed the conidial surface proteome of A. fumigatus, two closely related non-pathogenic species, Aspergillus fischeri and Aspergillus oerlinghausenensis, as well as pathogenic Aspergillus lentulus, to identify such proteins. After identifying 62 proteins exclusively detected on the A. fumigatus conidial surface, we assessed null mutants for 42 genes encoding these proteins. Deletion of 33 of these genes altered susceptibility to macrophage, epithelial cells and cytokine production. Notably, a gene that encodes a putative glycosylasparaginase, modulating levels of the host proinflammatory cytokine IL-1 beta, is important for infection in an immunocompetent murine model of fungal disease. These results suggest that A. fumigatus conidial surface proteins are important for evasion and modulation of the immune response at the onset of fungal infection. (AU) | |
| FAPESP's process: | 22/13603-4 - Large-scale data integration to study gene expression dynamics of Aspergillus fumigatus metabolic pathways related to caspofungin's antifungal action |
| Grantee: | Endrews Delbaje |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 22/08796-8 - Molecular characterization of putative effector proteins in the human fungal pathogen Aspergillus fumigatus |
| Grantee: | Camila Diehl da Rosa |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 20/04923-0 - SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 21/04977-5 - The identification of new pathways and compounds that can enhance caspofungin activity against Aspergillus fumigatus |
| Grantee: | Gustavo Henrique Goldman |
| Support Opportunities: | Research Projects - Thematic Grants |