Genomic Characterization of a Clinical NDM-1-Producing Klebsiella michiganensis from Brazil

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Yamada, Amanda Yaeko ; de Souza, Andreia Rodrigues ; Bertani, Amanda Maria de Jesus ; Campos, Karoline Rodrigues ; Sacchi, Claudio Tavares ; de Assis, Denise Brandao ; de Carvalho, Eneas ; Takagi, Elizabeth Harummyy ; Cunha, Marcos Paulo Vieira ; Tiba-Casas, Monique Ribeiro ; Camargo, Carlos Henrique

• Document type: Journal article
• Source: MICROORGANISMS; v. 12, n. 7, p. 10-pg., 2024-07-01.

Abstract

Public health faces daily challenges due to increasing reports of pathogenic microorganisms with new antimicrobial resistance. Klebsiella michiganensis, an emerging pathogen, poses difficulty in its identification using conventional techniques. This study presents the first documented case of NDM-1-producing K. michiganensis in Brazil, identified as the new ST418. Initially, the isolate from a tracheal secretion was misidentified as K. oxytoca. However, accurate identification was achieved through ANI analyses. Whole-genome sequencing was conducted to characterize the genetic context of the resistance genes, to identify virulence factors, and to construct a phylogenetic tree. The bla(NDM-1) gene was found to be harbored on an IncFIB plasmid approximately 112 kb in length, which was transferable in conjugation assays. The detection of carbapenem resistance genes in this species highlights the importance of public health vigilance, as it may serve as a reservoir and disseminator of significant resistance genes. (AU)

• FAPESP's process: 20/06157-2 - Genomic Epidemiology of NDM-producing enterobacteria from Brazil
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/11794-7 - Genomic and phylogenetic study of emerging strains of Acinetobacter baumannii belonging to Intenational Clone 2 during the COVID-19 pandemic period
• Grantee: Amanda Yaeko Yamada
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/50333-7 - Institutional research development plan of the Instituto Adolfo Lutz (PDIp)
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 18/21192-9 - Multi-User Equipment approved in grant 2017/50333-7: MALDI-TOF
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Multi-user Equipment Program