| Full text | |
| Author(s): Show less - |
Coletti, Thais de Moura
;
Romano, Camila Malta
;
Urbinatti, Paulo Roberto
;
Teixeira, Renildo Souza
;
de Almeida Pedrosa, Leila Weiss
;
Nardi, Marcello Schiavo
;
Natal, Delsio
;
da Costa, Antonio Charlys
;
de Jesus, Jaqueline Goes
;
Claro, Ingra Morales
;
Sabino, Ester Cerdeira
;
Witkin, Steven S.
;
Marrelli, Mauro Toledo
;
Fernandes, Licia Natal
Total Authors: 14
|
| Document type: | Journal article |
| Source: | Revista da Sociedade Brasileira de Medicina Tropical; v. 55, p. 5-pg., 2022-01-01. |
| Abstract | |
Background: Despite their worldwide occurrence, the distribution and role of insect-specific flaviviruses (ISFs) remain unclear. Methods: We evaluated the presence of ISFs in mosquitoes collected in Sao Paulo, Brazil, using reverse transcription and semi-nested polymerase chain reaction (PCR). Some of the positive samples were subjected to nanopore sequencing. Results: Twelve mosquito pools (2.8%) tested positive for flavivirus infection. Nanopore sequencing was successfully performed on six samples. Phylogenetic analysis grouped these sequences into genotype 2 of Culex flavivirus (CxFV). Conclusions: The identification of CxFV genotype 2 at new locations in Sao Paulo highlights the importance of understanding the role of ISFs in mosquito vector competence. (AU) | |
| FAPESP's process: | 18/14389-0 - Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE) |
| Grantee: | Ester Cerdeira Sabino |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/18204-0 - Flavivirus and Alphavirus identification in mosquitoes (Diptera: Culicidae) collected in green spaces of the city of São Paulo |
| Grantee: | Thaís de Moura Coletti |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/17176-8 - Real-time, rapid detection and sequencing of arboviruses in Brazil: Zika, Dengue, Chikungunya and Yellow Fever |
| Grantee: | Ingra Morales Claro |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 10/51230-8 - Biodiversity of mosquitoes (Diptera : Culicidae) in the city of São Paulo municipal parks |
| Grantee: | Mauro Toledo Marrelli |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 19/03859-9 - Clinical, serological and virological characteristics of Dengue virus serotype 2 (DENV-2) infection in a cohort previously exposed to serotype 1 |
| Grantee: | Camila Malta Romano |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/12000-1 - Modeling of the spatiotemporal dispersion of emergent arboviruses using heterogeneous data sources in the State of São Paulo |
| Grantee: | Jaqueline Goes de Jesus |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil |
| Grantee: | Antonio Charlys da Costa |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |