Confined Water Dynamics in the Scaffolds of Polylactic Acid

Author(s)

Ishikawa, Mariana ; Borges, Roger ; Mourao, Andre ; Ferreira, Leticie Mendonca ; Lobo, Anderson O. ; Martinho, Herculano

• Document type: Journal article
• Source: ACS OMEGA; v. 9, n. 18, p. 9-pg., 2024-04-23.

Abstract

Resorbable polylactic acid (PLA) ultrathin fibers have been applied as scaffolds for tissue engineering applications due to their micro- and nanoporous structure that favor cell adhesion, besides inducing cell proliferation and upregulating gene expression related to tissue regeneration. Incorporation of multiwalled carbon nanotubes into PLA fibers has been reported to increase the mechanical properties of the scaffold, making them even more suitable for tissue engineering applications. Ideally, scaffolds should be degraded simultaneously with tissue growth. Hydration and swelling are factors related to scaffold degradation. Hydration would negatively impact the mechanical properties since PLA shows hydrolytic degradation. Water absorption critically affects the catalysis and allowance of the hydrolysis reactions. Moreover, either mass transport and chemical reactions are influenced by confined water, which is an unexplored subject for PLA micro- and nanoporous fibers. Here, we probe and investigate confined water onto highly porous PLA microfibers containing few amounts of incorporated carbon nanotubes by Fourier transform infrared (FTIR) spectroscopy. A hydrostatic pressure was applied to the fibers to enhance the intermolecular interactions between water molecules and C=O groups from polyester bonds, which were evaluated over the wavenumber between 1600 and 2000 cm(-1). The analysis of temperature dependence of FTIR spectra indicated the presence of confined water which is characterized by a non-Arrhenius to Arrhenius crossover at T-0 = 190 K for 1716 and 1817 cm(-1) carbonyl bands of PLA. These bands are sensitive to a hydrogen bond network of confined water. The relevance of our finding relies on the challenge detecting confined water in hydrophobic cavities as in the PLA one. To the best of our knowledge, we present the first report referring the presence of confined water in a hydrophobic scaffold as PLA for tissue engineering. Our findings can provide new opportunities to understand the role of confined water in tissue engineering applications. For instance, we argue that PLA degradation may be affected the most by confined water. PLA degradation involves hydrolytic and enzymatic degradation reactions, which can both be sensitive to changes in water properties. (AU)

• FAPESP's process: 21/07490-0 - Salivary biomarkers of COVID-19 and other viral respiratory infections
• Grantee: Paulo Henrique Braz da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/02107-6 - The University of São Paulo twin panel: research on twins' behavior, health, and well-being
• Grantee: Emma Otta
• Support Opportunities: Research Projects - Thematic Grants