| Full text | |
| Author(s): |
de Freitas, Renato Leonardo
;
Acunha, Renata Moreira
;
Bendana-Cordoba, Fernando Rene
;
Medeiros, Priscila
;
Melo-Thomas, Liana
;
Coimbra, Norberto Cysne
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Brain Research; v. 1844, p. 19-pg., 2024-08-08. |
| Abstract | |
Rationale: The prelimbic division (PrL) of the medial prefrontal cortex (mPFC) is a key structure in panic. Objectives: To evaluate the role of nitric oxide (NO) in defensive behaviour and antinociception. Methods: Either N omega propyl-L-arginine (NPLA) or Carboxy-PTIO was microinjected in the PrL cortex, followed by hypothalamic treatment with bicuculline. The exploratory behaviours, defensive reactions and defensive antinociception were recorded. Encephalic c-Fos protein was immunolabelled after escape behaviour. Results: NPLA (an inhibition of nNOs) decreased panic-like responses and innate fear-induced antinociception. The c-PTIO (a membrane- impermeable NO scavenger) decreased the escape behaviour. PrL cortex pre-treatment with c-PTIO at all doses decreased defensive antinociception. c-Fos protein was labelled in neocortical areas, limbic system, and mesencephalic structures. Conclusion: The NPLA and c-PTIO in the PrL/mPFC decreased the escape behaviour and defensive antinociception organised by medial hypothalamic nuclei. The oriented escape behaviour recruits neocortical areas, limbic system, and mesencephalic structures. These findings suggest that the organisation of defensive antinociception recruits NO-signalling mechanisms within the PrL cortex. Furthermore, the present findings also support the role of NO as a retrograde messenger in the PrL cortex during panic-like emotional reactions. (AU) | |
| FAPESP's process: | 09/17258-5 - Study of the involvement of nitrergic system and of glutamatergic and cannabinoid-mediated neurotransmission from the medial prefrontal cortex in the analgesia induced by elaborated escape reactions evoked by GABAergic blockade in the medial hypothalamus |
| Grantee: | Renato Leonardo de Freitas |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/11855-8 - Study of corpus striatum and ventral mesencephalon endocanabinoid influence on neostriato-nigro-tectal GABAergic pathways during panic reactions of rodents threatened by venomous snakes |
| Grantee: | Norberto Cysne Coimbra |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/03798-0 - Involvement of opioid and endocanabinoid receptors of the substantia nigra on the activity of Nigro-Tectal GABAergic pathways during defensive behaviour elicited by rodents confronted with venomous snakes |
| Grantee: | Norberto Cysne Coimbra |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/25167-2 - Role of glutamatergic, endocannabinoid and endovaniloid systems of medial pre-frontal cortex in neurophatic pain model: Investigation of panic and chronic pain comorbidity |
| Grantee: | Priscila Medeiros de Freitas |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 07/01174-1 - Study of the Involvement of opioid-, serotonergic- and noradrenergic-mechanisms of the pain endogenous inhibitory system in antinociceptive processes induced by oriented escape reactions evoked by chemical stimulation of the merdial hypothalamus |
| Grantee: | Norberto Cysne Coimbra |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/11869-0 - Multi-use equipment approved in grant 2013/12916-0: deep brain stimulation (DBS) (Thomas mini matrix system - Thomas recording GmbH® - Winchester Strasse - Giessen - Germany) |
| Grantee: | Renato Leonardo de Freitas |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 03/05256-1 - Caracterizacao de receptores serotoninergicos na analgesia pos-ictal: envolvimento do nucleo dorsal da rafe. |
| Grantee: | Renato Leonardo de Freitas |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/12916-0 - Role of endocannabinoid, glutamatergic and endovanilloid systems of medial prefrontal cortex in neuropathic pain model and investigation of neurological disorders and chronic pain comorbidity |
| Grantee: | Renato Leonardo de Freitas |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/13560-5 - Effect of deep brain stimulation and cannabidiol treatment in the subthalamic nucleus on the motors and non-Motors symptoms in the experimental model of Parkinson Disease |
| Grantee: | Priscila Medeiros de Freitas |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 02/01497-1 - Estudo do envolvimento do núcleo magno da rafe, do núcleo gigantocelular e núcleo pré-tectal anterior, e de receptores opiódes, kappa e delta serotoninérgicos 5HT2/1C, e noradrenérgicos alfa, beta, na analgesia pós-cital: um estudo neuroanatômico e neuropsicofisiológico em um modelo experimental de epilepsia |
| Grantee: | Daoud Hibrahim Elias Filho |
| Support Opportunities: | Scholarships in Brazil - Technical Training Program - Technical Training |
| FAPESP's process: | 01/03752-6 - Estudo do envolvimento de receptores m-opioides, colinergicos muscarinico e nicotinico e serotonergicos 5-ht2/1c na analgesia pos-ictal. |
| Grantee: | Renato Leonardo de Freitas |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |