Bacterial Cellulose-Based Laser-Scribed Graphene Electrode for Hydrogen Peroxide Detection in Cancer Cells

Author(s)

de Lima, Lucas F. ; Ferreira, Andre L. ; dos Santos, Leticia Ester ; Coelho, Keyla Livian P. ; Santos, Keyla Teixeira ; Schmidt, Ariane ; de Jesus, Marcelo Bispo ; Paixao, Thiago R. L. C. ; de Araujo, William R.

• Document type: Journal article
• Source: ACS APPLIED BIO MATERIALS; v. 8, n. 7, p. 11-pg., 2025-06-29.

Abstract

The development of sustainable and high-performance electrochemical sensors is crucial for advancing biomedical applications. In this work, we introduce a hydrogen peroxide (H2O2) sensor based on bacterial cellulose-derived laser-scribed graphene (BC-LSG), modified with MXene and platinum nanoparticles (PtNPs). Bacterial cellulose (BC), a biodegradable and renewable material, was cultivated and transformed into a highly conductive carbon network using CO2 laser irradiation, producing a flexible, portable, and miniaturized electrochemical platform. The incorporation of MXene and PtNPs significantly enhanced the electrocatalytic response toward H2O2 oxidation, achieving a wide linear concentration range (15-95 mu mol L-1) and a low detection limit (0.35 mu mol L-1). Compared to traditional enzymatic sensors, our nanostructured BC-LSG device offers superior stability, reproducibility, and eco-friendliness, aligning with green analytical chemistry principles. The sensor was successfully applied for H2O2 detection in mammalian cells, demonstrating its potential for real-time monitoring of oxidative stress, a key biomarker in cancer progression and therapeutic responses. This work underscores the synergy between biopolymeric materials, nanotechnology, and laser processing, opening new avenues for scalable, disposable, and sustainable electrochemical devices. (AU)

• FAPESP's process: 23/00246-1 - Miniaturized large-scale devices for in-situ analysis: fabrication, characterization and applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/00723-1 - The role of IRF5 in the humoral response in emerging arboviroses with acute or chronic manifestations
• Grantee: Camila Lopes Simeoni
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 23/12589-0 - Fabrication of multiplex electrochemical (bio)sensors by CO2 laser engraving aiming the development of wearable devices.
• Grantee: Lucas Felipe de Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 23/08460-2 - Exsolution of Iridium Nanoparticles from Perovskite Oxides: searching for More Active and Stable Catalysts for the Production of Green Hydrogen
• Grantee: Keyla Teixeira Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 22/03250-7 - Development of portable and cost-effective biosensors for clinical diagnosis of bacterial and viral infections
• Grantee: William Reis de Araujo
• Support Opportunities: Research Grants - Initial Project