15d-PGJ2-loaded in nanocapsules enhance the antinociceptive properties into rat temporomandibular hypernociception

Author(s)

Clemente-Napimoga, Juliana T. ^[1] ; Moreira, Juscelaine A. ^[2] ; Grillo, Renato ^{[3, 4]} ; de Melo, Nathalie F. S. ^{[3, 4]} ; Fraceto, Leonardo F. ^{[3, 4]} ; Napimoga, Marcelo H. ^[5]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol, Lab Orofacial Pain, Campinas, SP - Brazil

^[2] Univ Uberaba, Lab Biopathol & Mol Biol, Uberaba, MG - Brazil

^[3] Univ Estadual Campinas, Dept Biochem, Campinas, SP - Brazil

^[4] Sao Paulo State Univ, Dept Environm Engn, Sao Paulo - Brazil

^[5] Sao Leopoldo Mand Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 90, n. 23-24, p. 944-949, JUN 14 2012.
• Web of Science Citations: 10

Abstract

Aims: To verify whether the nanoencapsulation of 15d-PGJ(2) in poly(D,L-lactide-co-glycolide) (PLGA) nanocapsules (15d-PGJ(2)-NC) might potentialize its antinociceptive activity into rats' temporomandibular joint (TMJ). Main methods: Transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to evaluate the morphology and suspension of the PLGA nanocapsules. Rats were pretreated (15 min) with an intra-TMJ injection of unloaded 15d-PGJ(2) or 15d-PGJ(2)-NC at concentrations of 10, 100 or 1000 pg followed by an ipsilateral intra-TMJ injection of 1.5% formalin. The nociceptive behavioral response was observed during 45 min: animals were then sacrificed and the periarticular tissue was removed for IL-1 beta measurements. Key finding: TEM and AFM analyses showed that 15d-PGJ(2)-NC is spherical without any aggregates or adhesion confirming that this formulation is a good drug carrier system for 15d-PGJ(2). Pretreatment with 15d-PGJ(2)-NC (100 and 1000 pg/TMJ), but not unloaded 15d-PGJ(2), was found to significantly decrease the release of IL-1 beta cytokine and the animals' nociceptive behavioral response induced by intra-TMJ injection of formalin. Significance: The compound 15d-PGJ(2)-NC might be used as a potential antinociceptive and anti-inflammatory agent to treat temporomandibular disorders in clinical practice. (c) 2012 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 10/15014-9 - Evaluation of the effect of 15d-PGJ2-loaded on polymeric biodegradable nanoparticles to control the evolution of the experimental periodontal disease
• Grantee: Marcelo Henrique Napimoga
• Support Opportunities: Regular Research Grants