Protection of Bifidobacterium lactis and Lactobacillus acidophilus by microencapsulation using spray-chilling

Author(s)

Pedroso, Daniela de Lara ^[1] ; Thomazini, Marcelo ^[1] ; Barrozo Heinemann, Riana Jordao ^[1] ; Favaro-Trindade, Carmen Silvia ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL DAIRY JOURNAL; v. 26, n. 2, p. 127-132, OCT 2012.
• Web of Science Citations: 56

Abstract

The aim of this study was to produce and evaluate solid lipid microparticles containing Bifidobacterium lactis or Lactobacillus acidophilus. Survival assays were conducted to evaluate the resistance of the probiotics to spray-chilling process, their resistance to simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) and their stability during 90 d of storage. The viability of the cells was not affected by microencapsulation. The free and encapsulated cells of B. lactis were resistant to SGF and SIF. The microencapsulation, however, provided protection for L. acidophilus against SGF and SIF. The free and encapsulated microorganisms lost their viability when they were stored at 37 degrees C. However, promising results were obtained when refrigerated and frozen storage was applied. The study indicates that spray-chilling using fat as carrier can be considered an innovative technology and matrix, respectively, for the protection, application and delivery of probiotics. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/11713-2 - Development of vehicles for controlled release of probiotics
• Grantee: Carmen Sílvia Fávaro Trindade
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/03140-2 - Production and evaluation of microcapsules of Lactobacillus acidophilus and Bifidobacterium lactis produced by spray chilling
• Grantee: Daniela de Lara Pedroso
• Support Opportunities: Scholarships in Brazil - Master