SUPERCRITICAL CARBON DIOXIDE SELECTIVITY TO FRACTIONATE PHENOLIC COMPOUNDS FROM THE DRY ETHANOLIC EXTRACT OF PROPOLIS

Author(s)

Paviani, Losiane C. ^[1] ; Dariva, Claudio ^[2] ; Marcucci, Maria C. ^[3] ; Cabral, Fernando A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Coll Food Engn, Lab Extracao Termodinam Aplicada & Equilibrio, Dept Engn Alimentos, Fac Engn Alimentos, BR-13083862 Sao Paulo - Brazil

^[2] Univ Tiradentes UNIT, BR-49031490 Aracaju, Sergipe - Brazil

^[3] Univ Bandeirante Sao Paulo UNIBAN, BR-02071013 Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF FOOD PROCESS ENGINEERING; v. 33, n. 1, p. 15-27, FEB 2010.
• Web of Science Citations: 21

Abstract

The global yield and composition of extracts obtained by supercritical carbon dioxide (SC-CO(2)) extraction from a dry ethanolic extract of propolis were measured in order to determine the possibility of using SC-CO(2) to fractionate components of interest present in these extracts. The global yield extraction was measured, and also the concentrations of the following phenolic compounds in the resulting supercritical fluid extracts (SFEs): 3,5-diprenyl-4-hydroxycinnamic acid (known as artepillin C), 3-prenyl-4-hydroxycinnamic acid, 4-hydroxycinnamic acid (p-coumaric acid) and 4-methoxy-3,5,7-trihydroxyflavone (kaempferide), of which artepillin C was the target component of greatest interest. The results showed extraction yields between 3.82 (at 150 bar) and 13.07% (at 350 bar), which could be highly correlated with the density of the SC-CO(2) at a constant temperature of 60C. The resulting concentrations in the SFE indicated that the selectivity of the carbon dioxide could be manipulated, and it was more selective at lower pressures, although with lower extraction yields. (AU)