| Full text | |
| Author(s): Show less - |
Kizys, Marina M. L.
[1]
;
Cardoso, Mirian G.
[1]
;
Lindsey, Susan C.
[1]
;
Harada, Michelle Y.
[1]
;
Soares, Fernando A.
[1]
;
Melo, Maria Clara C.
[1]
;
Montoya, Marlyn Z.
[1]
;
Kasamatsu, Teresa S.
[1]
;
Kunii, Ilda S.
[1]
;
Giannocco, Gisele
[1, 2]
;
Martins, Joao Roberto M.
[1, 3]
;
Cerutti, Janete M.
[4]
;
Maciel, Rui M. B.
[1]
;
Dias-da-Silva, Magnus R.
[1, 3]
Total Authors: 14
|
| Affiliation: | [1] Univ Fed Sao Paulo, Lab Mol & Translat Endocrinol, Dept Med, Unifesp, EPM, BR-04039032 Sao Paulo - Brazil
[2] Fac Med ABC FMABC, Dept Morphol & Physiol, Santo Andre, SP - Brazil
[3] Univ Fed Sao Paulo, EPM, Dept Biochem, Lab Mol & Translat Endocrinol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, EPM, Dept Morphol, Lab Mol & Translat Endocrinol, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Endocrinologia e Metabologia; v. 56, n. 9, p. 618-626, DEC 2012. |
| Web of Science Citations: | 23 |
| Abstract | |
OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26 (AU) | |
| FAPESP's process: | 11/20747-8 - Clinical, biochemical and molecular investigation of Thyrotoxic periodic paralysis |
| Grantee: | Magnus Régios Dias da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 06/60402-1 - Medular carcinoma of the thyroid: revisiting the clinical, molecular biological, biochemical and biological aspects following findings of molecular genetics |
| Grantee: | Rui Monteiro de Barros Maciel |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 10/19834-0 - Methylation pattern characterization and RET (MAPK), HES1 (Notch) and WNT5A (WNT/ß-CATENINA) gene expression in medullary thyroid carcinoma cell line (TT) |
| Grantee: | Mirian Gonçalves Cardoso |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |