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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial Cyanopeptide Action on Bacterial Cells Observed with Atomic Force Microscopy

Full text
Author(s):
Silva-Stenico, Maria Estela [1] ; Lorenzi, Adriana Sturion [1] ; Teschke, Omar [2] ; Pamplona Silva, Caroline Souza [1] ; Etchegaray, Augusto [3] ; Fiore, Marli Fatima [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Mol Ecol Cyanobacteria Lab, BR-13400970 Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, Inst Phys, NanoStruct & Interfaces Lab, BR-13083970 Campinas, SP - Brazil
[3] Pontifical Catholic Univ Campinas, Fac Chem, BR-13012970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CURRENT NANOSCIENCE; v. 9, n. 1, p. 141-148, FEB 2013.
Web of Science Citations: 1
Abstract

Cyanobacteria produce oligopeptides that are predominantly synthesized by the non-ribosomal pathway. Among these are the aeruginosin and cyanopeptolin protease inhibitors, which act against enzymes known to cause several human health problems. Atomic force microscopy (AFM) was used to study the effect of cyanopeptides produced by Microcystis aeruginosa NPCD-1 on pathogenic bacterial cell surfaces. The selected strain was characterized based on the 16S rRNA gene sequence and the intergenic spacer region of the phycocyanin operon. PCR amplification was employed to investigate the presence of genes encoding for aeruginosin and cyanopeptolin. Purified extract from M. aeruginosa NPCD-1 cells was screened for bioactive compounds. The effect of purified extract containing protease inhibitors produced by the NPCD-1 strain on bacterial cells was observed using AFM. Aeruginosin and cyanopeptolin genes were confirmed by both PCR amplification and gene sequencing. Mass spectrometry analysis confirmed the production of aeruginosin. The interaction of Bacillus cereus, Escherichia coli and Staphylococcus aureus with cyanopeptides was characterized by examining the loss of surface stiffness and the formation of micelles, most likely originating from the membrane disruption. The AFM results demonstrate the ability of cyanobacterial extract to alter the cellular membrane of bacterial pathogens. (AU)

FAPESP's process: 06/01671-2 - Molecular characterization of cyanobacteria strains isolated from São Paulo State mangrove regions
Grantee:Caroline Souza Pamplona da Silva
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/05474-5 - Bioprospection of cyanobacteria: screening cyanopeptides for pharmaceutical and biotechnological applications
Grantee:Maria Estela Stenico
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 10/09867-9 - Bioprospection of cyanobacteria: screening cyanopeptides for pharmaceutical and biotechnological applications
Grantee:Maria Estela Stenico
Support Opportunities: Scholarships in Brazil - Young Researchers
FAPESP's process: 03/12529-4 - Nanotechnology: manufacture of nanostructures and supramolecular structures in liquid medium: observation of structures formed and investigation of processes of formation of nanostructures
Grantee:Omar Teschke
Support Opportunities: Research Projects - Thematic Grants