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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo protective effect of Copaifera langsdorffii hydroalcoholic extract on micronuclei induction by doxorubicin

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Author(s):
Alves, Jacqueline Morais [1] ; Munari, Carla Carolina [1] ; Bentes Monteiro Neto, Moacir de Azevedo [1] ; Furtado, Ricardo Andrade [2] ; Senedese, Juliana Marques [1] ; Bastos, Jairo Kenupp [2] ; Tavares, Denise Crispim [1]
Total Authors: 7
Affiliation:
[1] Univ Franca, BR-14404600 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF APPLIED TOXICOLOGY; v. 33, n. 8, p. 854-860, AUG 2013.
Web of Science Citations: 21
Abstract

Copaifera lansdorffii Desf. is known as copaiba', copaiva' or pau-de-oleo', and is found in part of Brazil. The present study was undertaken to evaluate the genotoxic potential of C. langsdorffii leaf hydroalcoholic extract (CLE) and its influence on the genotoxicity induced by the chemotherapeutic agent doxorubicin (DXR) using the Swiss mouse peripheral blood micronucleus test. HPLC analysis of CLE using two monolithic columns linked in series allowed quantification of two major flavonoid heterosides, quercitrin and afzelin. Animals were treated with CLE by gavage at doses of 10, 20, 40 and 80mgkg-1 body weight per day, each for 20days. Peripheral blood samples were collected at 24 and 48h, and 7, 15 and 21days after the beginning of the treatment. For the antigenotoxicity evaluation, the animals treated with different concentrations of CLE received DXR (15mgkg-1 body weight, intraperitoneal) at day 20. The peripheral blood samples were collected 24 and 48h after the treatment with DXR. The results demonstrated that CLE itself was not genotoxic in the mouse micronucleus assay. In animals treated with CLE and DXR, the number of micronucleus was significantly decreased compared with animals receiving DXR alone. The putative antioxidant activity of one or more of the active compounds of CLE may explain the effect of this plant on DXR genotoxicity. Copyright (c) 2012 John Wiley \& Sons, Ltd. (AU)