Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Small-angle X-ray scattering and structural modeling of full-length: cellobiohydrolase I from Trichoderma harzianum

Full text
Author(s):
Lima, Leonardo H. F. [1] ; Serpa, Viviane I. [1] ; Rosseto, Flavio R. [1] ; Sartori, Geraldo Rodrigues [1] ; de Oliveira Neto, Mario [1] ; Martinez, Leandro [1] ; Polikarpov, Igor [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Grp Biotecnol Mol, IFSC, BR-13566570 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Cellulose; v. 20, n. 4, p. 1573-1585, AUG 2013.
Web of Science Citations: 7
Abstract

Cellobiohydrolases hydrolyze cellulose releasing cellobiose units. They are very important for a number of biotechnological applications, such as, for example, production of cellulosic ethanol and cotton fiber processing. The Trichoderma cellobiohydrolase I (CBH1 or Cel7A) is an industrially important exocellulase. It exhibits a typical two domain architecture, with a small C-terminal cellulose-binding domain and a large N-terminal catalytic core domain, connected by an O-glycosylated linker peptide. The mechanism by which the linker mediates the concerted action of the two domains remains a conundrum. Here, we probe the protein shape and domain organization of the CBH1 of Trichoderma harzianum (ThCel7A) by small angle X-ray scattering (SAXS) and structural modeling. Our SAXS data shows that ThCel7A linker is partially-extended in solution. Structural modeling suggests that this linker conformation is stabilized by inter- and intra-molecular interactions involving the linker peptide and its O-glycosylations. (AU)

FAPESP's process: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
Grantee:Igor Polikarpov
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 07/08706-9 - Molecular and structural studies of Cellulitic complex enzymes of fungi Trichoderma harzianum with potencial of enzymatic transformation of biomass
Grantee:Viviane Isabel Serpa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/16947-9 - Correlations between dynamics, structure and function in protein: computer simulations and algorithms
Grantee:Leandro Martinez
Support type: Regular Research Grants
FAPESP's process: 09/52840-7 - Center of Biological and Industrial Processes for Biofuels - CeProBIO
Grantee:Igor Polikarpov
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 08/05637-9 - Structural studies of the three isotypes of the peroxisomal proliferator-activated receptor hPPAR and them interaction with retinoid receptor hRXRalfa using small angle X-ray scattering
Grantee:Mario de Oliveira Neto
Support type: Scholarships in Brazil - Post-Doctorate