| Full text | |
| Author(s): |
Lima, Leonardo H. F.
[1]
;
Serpa, Viviane I.
[1]
;
Rosseto, Flavio R.
[1]
;
Sartori, Geraldo Rodrigues
[1]
;
de Oliveira Neto, Mario
[1]
;
Martinez, Leandro
[1]
;
Polikarpov, Igor
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Grp Biotecnol Mol, IFSC, BR-13566570 Sao Carlos, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Cellulose; v. 20, n. 4, p. 1573-1585, AUG 2013. |
| Web of Science Citations: | 7 |
| Abstract | |
Cellobiohydrolases hydrolyze cellulose releasing cellobiose units. They are very important for a number of biotechnological applications, such as, for example, production of cellulosic ethanol and cotton fiber processing. The Trichoderma cellobiohydrolase I (CBH1 or Cel7A) is an industrially important exocellulase. It exhibits a typical two domain architecture, with a small C-terminal cellulose-binding domain and a large N-terminal catalytic core domain, connected by an O-glycosylated linker peptide. The mechanism by which the linker mediates the concerted action of the two domains remains a conundrum. Here, we probe the protein shape and domain organization of the CBH1 of Trichoderma harzianum (ThCel7A) by small angle X-ray scattering (SAXS) and structural modeling. Our SAXS data shows that ThCel7A linker is partially-extended in solution. Structural modeling suggests that this linker conformation is stabilized by inter- and intra-molecular interactions involving the linker peptide and its O-glycosylations. (AU) | |
| FAPESP's process: | 09/52840-7 - Center of Biological and Industrial Processes for Biofuels - CeProBIO |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 07/08706-9 - Molecular and Structural Studies of Cellulitic Complex Enzymes of Fungi Trichoderma harzianum with Potencial of Enzymatic Transformation of Biomass |
| Grantee: | Viviane Isabel Serpa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 08/05637-9 - Structural studies of the three isotypes of the peroxisomal proliferator-activated receptor hPPAR and them interaction with retinoid receptor hRXRalfa using small angle X-ray scattering |
| Grantee: | Mario de Oliveira Neto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/16947-9 - Correlations between dynamics, structure and function in protein: computer simulations and algorithms |
| Grantee: | Leandro Martinez |
| Support Opportunities: | Regular Research Grants |