Evaluation of fluoride release from experimental TiF4 and NaF varnishes in vitro

Author(s)

Livia Picchi COMAR ^[1] ; Beatriz Martines de SOUZA ^[1] ; Larissa Tercilia GRIZZO ^[1] ; Marília Afonso Rabelo BUZALAF ^[4] ; Ana Carolina MAGALHÃES ^[5]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[3] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 22, n. 2, p. 138-143, 2014-04-00.

Abstract

Fluoride varnishes play an important role in the prevention of dental caries, promoting the inhibition of demineralization and the increase of remineralization. Objective: This study aimed to analyze the amount of fluoride released into water and artificial saliva from experimental TiF4 and NaF varnishes, with different concentrations, for 12 h. Material and Methods: Fluoride varnishes were applied on acrylic blocks and then immersed in 10 ml of deionized water and artificial saliva in polystyrene bottles. The acrylic blocks were divided in seven groups (n=10): 1.55% TiF4 varnish (0.95% F, pH 1.0); 3.10% TiF4 varnish (1.90% F, pH 1.0); 3.10% and 4% TiF4 varnish (2.45% F, pH 1.0); 2.10% NaF varnish (0.95% F, pH 5.0); 4.20% NaF varnish (1.90% F, pH 5.0); 5.42% NaF varnish (2.45% F, pH 5.0) and control (no treatment, n=5). The fluoride release was analyzed after 1/2, 1, 3, 6, 9 and 12 h of exposure. The analysis was performed using an ion-specific electrode coupled to a potentiometer. Two-way ANOVA and Bonferroni's test were applied for the statistical analysis (p<0.05). Results: TiF4 varnishes released larger amounts of fluoride than NaF varnishes during the first 1/2 h, regardless of their concentration; 4% TiF4 varnish released more fluoride than NaF varnishes for the first 6 h. The peak of fluoride release occurred at 3 h. There was a better dose-response relationship among the varnishes exposed to water than to artificial saliva. Conclusions: The 3.10% and 4% TiF4 -based varnishes have greater ability to release fluoride into water and artificial saliva compared to NaF varnish; however, more studies must be conducted to elucidate the mechanism of action of TiF4 varnish on tooth surface. (AU)

• FAPESP's process: 12/20698-0 - Effect of TiF4 on hydroxiapatite and enamel subjected to cariogenic and erosive processes: in vitro and in situ studies
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/07001-4 - Evaluation of the effect of an experimental paste with calcium phosphate nanoparticles on enamel demineralization in vitro
• Grantee: Beatriz Martines de Souza
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/11263-7 - Evaluation of TiF4 interaction with hydroxyapatite and enamel and the effect on the de-remineralization of artificial caries enamel lesion in situ
• Grantee: Livia Picchi Comar
• Support Opportunities: Scholarships in Brazil - Doctorate