Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the methylation profile of exfoliated cell samples from patients with head and neck squamous cell carcinoma

Full text
Author(s):
Bomfim Longo, Ana Luiza [1] ; Rettori, Marianna M. [1] ; de Carvalho, Ana Carolina [1] ; Kowalski, Luiz Paulo [2] ; Carvalho, Andre Lopes [3] ; Vettore, Andre Luiz [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Dept Ciencias Biol, Lab Biol Mol Canc, Sao Paulo - Brazil
[2] Hosp AC Camargo Fund Antonio Prudente, Dept Cirurgia Cabeca & Pescoco, Sao Paulo - Brazil
[3] Hosp Canc Barretos, Dept Cirurgia Cabeca & Pescoco, Barretos - Brazil
Total Affiliations: 3
Document type: Journal article
Source: HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK; v. 36, n. 5, p. 631-637, MAY 2014.
Web of Science Citations: 6
Abstract

Background Silencing of tumor suppressor genes plays a vital role in head and neck carcinogenesis. The purposes of this study were to determine the methylation profile of exfoliated tumors cells collected from patients with head and neck squamous cell carcinoma (HNSCC) and to evaluate its prognostic significance. Methods The methylation profile and level of a 20-gene panel were evaluated by quantitative methylation-specific polymerase chain reaction (qMSP) in exfoliated tumor cell samples from 96 patients with HNSCC. Results CCNA1 (60.4%), DCC (54.2%), and TIMP3 (35.4%) were frequently methylated in these samples. Patients with exfoliated tumors cells positive for DCC methylation showed a trend toward a lower local recurrence-free survival. Conclusion These findings indicate that a low invasive method could be used to access the methylation profile of exfoliated cells from patients with HNSCC. Moreover, our data provide evidence that hypermethylation of DCC could be useful as prognostic indicator for this malignancy. (c) 2013 Wiley Periodicals, Inc. Head Neck 36: 631-637, 2014 (AU)

FAPESP's process: 05/02580-8 - Evaluation of methylation profile as prognostic marker in patients with head and neck tumors
Grantee:Andre Luiz Vettore de Oliveira
Support Opportunities: Regular Research Grants