Interaction of chitosans and surfactants with cell membrane models investigated by nonlinear vibrational spectroscopy

• Grant number: 18/02819-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: November 30, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Paulo Barbeitas Miranda
• Grantee: Paulo Barbeitas Miranda
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

Chitosan, an abundant natural polymer, has been used in many biological applications such as drug delivery, gene therapy, tissue engineering and antimicrobial systems. Although these applications have grown greatly in recent years, there are several models for the modes of interaction at the molecular level of chitosan with biological membranes. Therefore, interactions between chitosan derivatives and membrane models of mammals, bacteria and fungi will be investigated in this work. Langmuir films of lipids will be used as cell membrane models, with the novelty that biologically relevant temperature will be used in the experiments in order to allow extrapolating the obtained results to in vitro or in vivo situations. Sum-Frequency Generation vibrational spectroscopy (SFG spectroscopy) will provide information about the interfacial order of water molecules, the interaction of chitosan with the polar group of lipids, the conformation of lipid chains and chitosan penetration in the membrane models. Sum-Frequency Generation microscopy (SFG microscopy), an innovative technique that has recently been implemented in our labs, will be used to obtain images at interfaces with chemical sensitivity by the vibrational spectrum and contrast by the orientation and ordering of the molecules. The techniques of polarization-modulated infrared reflection spectroscopy (PM-IRRAS) and Brewster angle microscopy (BAM) will be used for comparison with results from SFG spectroscopy and microscopy. The results are expected to provide a solid basis for understanding the role of positive charges, degree of hydrophobicity, and hydrogen bonds in the interactions between chitosan and membrane models. We also plan to use the same methods to investigate the interaction between synthetic semi-fluorinated surfactants with membrane phospholipids, as a model system for the action of lung surfactants. Therefore, this work will contribute to elucidate the mechanism of molecular interaction of chitosan derivatives and surfactants with cellular membranes of mammals, bacteria and fungi, using powerful techniques that are not widely used in this area. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUSAIN, ABHAY; FARIA, ROBERTO M.; MIRANDA, PAULO B.. Polymer Solar Cells-Interfacial Processes Related to Performance Issues. FRONTIERS IN CHEMISTRY, v. 7, FEB 12 2019. (17/07635-2, 18/02819-0)

RIMOLI, CAIO VAZ; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.. Interaction mechanism of chitosan oligomers in pure water with cell membrane models studied by SFG vibrational spectroscopy. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 219, p. 12-pg., 2022-09-02. (18/02819-0, 13/23100-0, 14/01595-0, 17/03503-4)

CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, p. 7-pg., 2020-04-01. (15/12990-0, 12/15543-7, 18/02819-0, 13/14262-7, 16/01919-6, 19/13390-8, 17/03503-4)

PEDRO, RAFAEL DE OLIVEIRA; PEREIRA, ANDRESSA RIBEIRO; OLIVEIRA, OSVALDO N.; MIRANDA, PAULO BARBEITAS. Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 192, AUG 2020. (18/00878-0, 18/02819-0, 17/03503-4)

ARJUNAN, ANANTHI; SUKERI, ANANDHAKUMAR; SARAIVA, DOUGLAS PHILIP MARTINEZ; MIRANDA, PAULO BARBEITAS; BERTOTTI, MAURO. Electrochemical Studies of Hydrogen Peroxide Oxidation on a Nanoporous Gold Surface: Fundamental and Analytical Applications. Journal of the Electrochemical Society, v. 167, n. 11, JAN 8 2020. (18/08782-1, 18/02819-0)

CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, APR 1 2020. (17/03503-4, 12/15543-7, 18/02819-0, 15/12990-0, 13/14262-7, 19/13390-8, 16/01919-6)

RAYMUNDO-PEREIRA, PAULO A.; PEDRO, RAFAEL DE OLIVEIRA; CARR, OLIVIA; MELENDEZ, MATIAS E.; GOBBI, ANGELO L.; PIAZZETTA, MARIA HELENA DE OLIVEIRA; CARVALHO, ANDRE L.; REIS, RUI M.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3. Journal of Physical Chemistry C, v. 125, n. 1, p. 498-506, JAN 14 2021. (16/01919-6, 17/03503-4, 18/22214-6, 18/02819-0)

UEHARA, THIERS MASSAMI; CANCINO-BERNARDI, JULIANA; MIRANDA, PAULO BARBEITAS; ZUCOLOTTO, VALTENCIR. Investigating the interactions of corona-free SWCNTs and cell membrane models using sum-frequency generation. SOFT MATTER, v. 16, n. 24, p. 5711-5717, JUN 28 2020. (12/03570-0, 18/02819-0, 10/00486-2)

UEHARA, THIERS M.; MIGLIORINI, FERNANDA L.; FACURE, MURILO H. M.; PALMA FILHO, NICOLAU B.; MIRANDA, PAULO B.; ZUCOLOTTO, VALTENCIR; CORREA, DANIEL S.. anostructured scaffolds containing graphene oxide for nanomedicine application. POLYMERS FOR ADVANCED TECHNOLOGIES, v. 33, n. 2, NOV 2021. (17/12174-4, 17/10582-8, 18/02819-0, 17/21791-7, 18/22214-6)

GUSAIN, ABHAY; FARIA, ROBERTO M.; MIRANDA, PAULO B.. Polymer Solar Cells-Interfacial Processes Related to Performance Issues. RONTIERS IN CHEMISTR, v. 7, p. 25-pg., 2019-02-12. (18/02819-0, 17/07635-2)

Filed patent(s) as a result of this research project