Multi-user equipment approved in grant 2017/04138-8 for high pressure semipreparative chromatographic system model LC-20AR

• Grant number: 18/05080-6
• Support Opportunities: Multi-user Equipment Program
• Start date: May 01, 2018
• End date: April 30, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Sergio Ricardo Ambrosio
• Grantee: Sergio Ricardo Ambrosio
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM
• EMU web page: https://www.unifran.edu.br/pos-graduacao-pesquisa-extensao/mestrado-e-doutorado/ciencias/o-programa/apresentacao
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
• Manufacturer: Shimadzu
• Model: LC-20AR

Abstract

The Brazilian propolis produced by Apis mellifera called green, brown and red have great economic importance, due to their biological activities and export to several countries, such as Japan and China, and production of approximately 200 tons per year. The main botanical origins of Brazilian propolis are: Baccharis dracunculifolia for green propolis; Araucaria sp, Eucalyptus sp and others for brown propolis; and Dalbergia ecastophyllum for the red propolis. In 2005, ANVISA published a technical note on the registration of products containing propolis, as well as the RDC no. 24/2011 authorizing the registration of propolis-based medicines. However, to date none medicine has been registered with ANVISA. There are many gaps to be filled, since ANVISA demands the execution of chemical, analytical, pharmacological and technological studies to guarantee safety, efficacy and quality in the development of new medicines. Thus, it is proposed to carry out complementary steps in phytochemical studies to obtain standardized extracts and compounds to be used in the development of analytical methods; in obtaining semi-synthetic derivatives; in stability and degradation studies; in bioaccessibility, biotransformation, pharmacokinetic and pharmacodynamic studies; in silico studies to determine chemical structure-activity relationship and docking analyses; in the development of pharmaceutical formulations; in both in vitro and in vivo toxicological studies; in studies of genotoxicity and mutagenicity; in anti-inflammatory, immunomodulatory, antimicrobial, antiparasitic, antiproliferative, healing and gastro protective assays, among others. The ultimate goal of this proposal is to add value to the Brazilian beekeeping sector. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, THAYNA DE SOUZA; SILVA, JULIA M. B.; BRAUN, GLAUCIA H.; MEJIA, JENNYFER A. A.; CCAPATINTA, GARI V. C.; CONCEICAO SANTOS, MARIO FERREIRA; TANIMOTO, MATHEUS H.; BASTOS, JAIRO K.; PARREIRA, RENATO L. T.; ORENHA, RENATO P.; et al. Green and Red Brazilian Propolis: Antimicrobial Potential and Anti-Virulence against ATCC and Clinically Isolated Multidrug-Resistant Bacteria. CHEMISTRY & BIODIVERSITY, JUN 2021. (19/01697-1, 18/05080-6, 18/13700-4, 17/04138-8)

SANTOS, MARIO FERREIRA CONCEICAO; OLIVEIRA, LARISSA COSTA; RIBEIRO, VICTOR PENA; SOARES, MARISI GOMES; MORAE, GABRIEL DE OLIVEIRA ISAC; SARTORI, ALAN GIOVANINI DE OLIVEIRA; ROSALEN, PEDRO LUIZ; BASTOS, JAIRO KENUPP; DE ALENCAR, SEVERINO MATIAS; VENEZIANI, RODRIGO CASSIO SOLA; et al. Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high-performance liquid chromatography method for its analysis. JOURNAL OF SEPARATION SCIENCE, v. 44, n. 16, JUL 2021. (17/04138-8, 18/05080-6, 18/25010-2)

PAULA, LUCAS A. DE L.; SANTOS, MARIO F. C.; PAGOTTI, MARIANA C.; VENEZIANI, RODRIGO C. S.; BASTOS, JAIRO K.; CAFFREY, CONOR R.; AMBROSIO, SERGIO R.; MAGALHAES, LIZANDRA G.. razilian green propolis reduces worm burden and hepatic granuloma formation in a Schistosoma mansoni experimental murine mode. Parasitology Research, v. 121, n. 2, p. 775-780, FEB 2022. (18/50011-2, 17/04138-8, 18/25010-2, 18/05080-6)

E SILVA, MARCIO L. A.; LUCARINI, RODRIGO; DOS SANTOS, FRANSERGIO F.; MARTINS, CARLOS H. G.; PAULETTI, PATRICIA M.; JANUARIO, ANA H.; CONCEICAO SANTOS, MARIO FERREIRA; CUNHA, WILSON R.. Hypoglycemic effect of rosmarinic acid-rich infusion (RosCE) from Origanum vulgare in alloxan-induced diabetic rats. NATURAL PRODUCT RESEARCH, OCT 2021. (18/25010-2, 18/05080-6)

LIMA, THAIS C.; MAGALHAES, LIZANDRA G.; PAULA, LUCAS A. DE L.; CUNHA, WILSON R.; JANUARIO, ANA H.; PAULETTI, PATRICIA M.; BASTOS, JAIRO K.; MNUQUIAN, HALLANA ALVES; FORIM, MOACIR R.; MORAIS-URANO, RAQUEL P.; et al. In vivo schistosomicidal activity of (+/-)-licarin A-loaded poly (epsilon-caprolactone) nanoparticles. Experimental Parasitology, v. 241, p. 7-pg., 2022-09-01. (18/05080-6, 18/25010-2, 10/17378-8, 09/15207-4, 11/07623-8, 11/05952-4)

SILVA, THAYNA DE SOUZA; SILVA, JULIA M. B.; BRAUN, GLAUCIA H.; MEJIA, JENNYFER A. A.; CCAPATINTA, GARI V. C.; CONCEICAO SANTOS, MARIO FERREIRA; TANIMOTO, MATHEUS H.; BASTOS, JAIRO K.; PARREIRA, RENATO L. T.; ORENHA, RENATO P.; et al. Green and Red Brazilian Propolis: Antimicrobial Potential and Anti-Virulence against ATCC and Clinically Isolated Multidrug-Resistant Bacteria. CHEMISTRY & BIODIVERSITY, v. 18, n. 8, AUG 2021. (18/13700-4, 17/04138-8, 19/01697-1, 18/05080-6)

BARBOSA, LORRANA VIETRO; CASTRO, MONA STEFANY DE SOUZA; CIUFFI, KATIA JORGE; NASSAR, EDUARDO J.; MARCAL, LIZIANE; PEREIRA, LORENA RODRIGUES; SANTOS, MARIO FERREIRA CONCEICAO; AMBROSIO, SERGIO RICARDO; GOMES, PIETRA STEFANY DA SILVA; NOGUEIRA, FLAVIA APARECIDA RESENDE; et al. Kaolinite-titanium nanocomposite applied to the photodegradation of the genotoxic compound cisplatin and the terephthalic acid hydroxylation reaction. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 676, p. 12-pg., 2023-11-05. (17/15482-1, 18/05080-6, 18/25010-2, 11/09823-4, 17/16278-9, 11/09823-4, 19/26439-5, 20/06712-6, 16/01501-1)