Development of glucose biosensor as an analytical tool aiming the optimization of second-generation ethanol production from pineapple residues

• Grant number: 17/24274-3
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: February 01, 2019
• End date: April 30, 2021
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Ivana Cesarino
• Grantee: Ivana Cesarino
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• Associated researchers: Alcides Lopes Leao

Abstract

Bioethanol is a fuel derived from renewable energy sources. However, the main production processes use biomasses that are also used also as food. For this reason, new technologies are needed that use alternative sources as a raw material, such as the production of second-generation (2G) ethanol, which uses lignocellulosic biomass in its process. This biomass can be in the form of bagasse, straw, bark, in other words, products that are residues from the agricultural chain production. In addition, these residues can be plants that in some way cause problems (eg. weeds), like the water hyacinth that in a short time can cover water bodies, causing its eutrophication. An example of agricultural waste that may contribute toward is the pineapple residues. Is a vegetal biomass and contains in its structure cellulose that can be degraded into glucose and later used as substrate for the production of ethanol. However, during the enzymatic hydrolysis of cellulose, glucose becomes a limiting factor, since its accumulation in the bioreactor causes inhibition in the cellulase enzyme, and this implies in a lower production of this hexose consequently a lower yield in ethanol production. Therefore, it is proposed the development of sensors associated with the electrochemical potential of graphene and the specificity of glucose enzyme for the monitoring and quantification of glucose in the 2G ethanol production process. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SETZNAGL, SARAH; CESARINO, IVANA. Copper nanoparticles and reduced graphene oxide modified a glassy carbon electrode for the determination of glyphosate in water samples. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, FEB 2020. (17/24274-3)

BRONZATO, GIOVANA R. F.; DOS REIS, VICTOR A. C. A.; BORRO, JESSYCA A.; LEAO, ALCIDES L.; CESARINO, IVANA. Second generation ethanol made from coir husk under the biomass Cascade approach. MOLECULAR CRYSTALS AND LIQUID CRYSTALS, v. 693, n. 1, SI, p. 107-114, NOV 2 2019. (17/24274-3)

SILVA, MARTIN K. L.; CESARINO, IVANA. Electrochemical sensor based on Sb nanoparticles/reduced graphene oxide for heavy metal determination. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, MAY 2020. (17/24274-3)

NUNES, ERIK W.; SILVA, MARTIN K. L.; CESARINO, IVANA. Evaluation of a Reduced Graphene Oxide-Sb Nanoparticles Electrochemical Sensor for the Detection of Cadmium and Lead in Chamomile Tea. CHEMOSENSORS, v. 8, n. 3, p. 12-pg., 2020-09-01. (18/09053-3, 17/24274-3)

ZACCARIOTTO, GABRIEL C.; SILVA, MARTIN K. L.; ROCHA, GIOVANNA S.; CESARINO, IVANA. Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosenso. MATERIALS, v. 14, n. 15, AUG 2021. (17/24274-3)

TRINDADE, CLARA M. B.; SILVA, MARTIN K. L.; CESARINO, IVANA. Copper nanostructures anchored on renewable carbon as electrochemical platform for the detection of dopamine, fluoxetine and escitalopram. SENSORS AND ACTUATORS REPORTS, v. 4, p. 8-pg., 2022-11-01. (17/24274-3)

DONINI, CARUANE A.; SILVA, MARTIN K. L.; BRONZATO, GIOVANA R.; LEAO, ALCIDES L.; CESARINO, IVANA. Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production. Journal of Solid State Electrochemistry, v. 24, n. 8, p. 8-pg., 2019-12-23. (17/24274-3, 17/03925-6)

BRONZATO, GIOVANA R. F.; DOS REIS, VICTOR A. C. A.; BORRO, JESSYCA A.; LEAO, ALCIDES L.; CESARINO, IVANA. Second generation ethanol made from coir husk under the biomass Cascade approach. MOLECULAR CRYSTALS AND LIQUID CRYSTALS, v. 693, n. 1, p. 8-pg., 2019-11-02. (17/24274-3)

NUNES, ERIK W.; SILVA, MARTIN K. L.; RASCON, JESUS; LEIVA-TAFUR, DAMARIS; LAPA, RAINER M. L.; CESARINO, IVANA. Acetylcholinesterase Biosensor Based on Functionalized Renewable Carbon Platform for Detection of Carbaryl in Food. BIOSENSORS-BASEL, v. 12, n. 7, p. 14-pg., 2022-07-01. (19/19578-9, 17/24274-3)

DONINI, CARUANE A.; SILVA, MARTIN K. L.; BRONZATO, GIOVANA R.; LEAO, ALCIDES L.; CESARINO, IVANA. Evaluation of a biosensor based on reduced graphene oxide and glucose oxidase enzyme on the monitoring of second-generation ethanol production. Journal of Solid State Electrochemistry, v. 24, n. 8, SI, DEC 2019. (17/03925-6, 17/24274-3)

FERNANDES, VICTORIA QUINTAS; LEME SILVA, MARTIN K.; CESARINO, IVANA. Determination of isotretinoin (13-cis-retinoic acid) using a sensor based on reduced graphene oxide modified with copper nanoparticles. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 856, JAN 1 2020. (17/24274-3, 17/08733-8)

SILVA, MARTIN K. L.; SOUSA, GUILHERME S.; SIMOES, RAFAEL P.; CESARINO, IVANA. Fabrication of paper-based analytical devices using a PLA 3D-printed stencil for electrochemical determination of chloroquine and escitalopram. Journal of Solid State Electrochemistry, v. 26, n. 2, NOV 2021. (17/24274-3)

SIRLEY REIS TEIXEIRA, WANDERSON; KASSIO LEME SILVA, MARTIN; GRASSESCHI, DANIEL; SENNA, CARLOS ALBERTO; DE OLIVEIRA, ANDRE GUIMARAES; GRUBER, JONAS; CESARINO, IVANA; SALLES, MAIARA OLIVEIRA. Principal Component Analysis as a Tool for Electrochemical Characterization of Modified Electrodes: A Case Study. Journal of the Electrochemical Society, v. 169, n. 4, p. 9-pg., 2022-04-01. (17/24274-3, 15/02136-2)

SILVA, MARTIN K. L.; SIMOES, RAFAEL P.; LEAO, ALCIDES L.; LAPA, RAINER M. L.; RASCON, JESUS; CESARINO, IVANA. Competitive Host-guest Electrochemical Detection of Ivermectin Drug Using a beta-cyclodextrin/Graphene-based Electrode. Electroanalysis, v. N/A, p. 13-pg., 2022-05-31. (17/24274-3)

SETZNAGL, SARAH; CESARINO, IVANA. Copper nanoparticles and reduced graphene oxide modified a glassy carbon electrode for the determination of glyphosate in water samples. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, v. 102, n. 1, p. 293-305, JAN 2 2022. (17/24274-3)

SILVA, MARTIN K. L.; CESARINO, IVANA. Electrochemical sensor based on Sb nanoparticles/reduced graphene oxide for heavy metal determination. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, v. 102, n. 13, p. 15-pg., 2020-05-15. (17/24274-3)

SILVA, MARTIN; CESARINO, IVANA. Evaluation of a Nanocomposite Based on Reduced Graphene Oxide and Gold Nanoparticles as an Electrochemical Platform for Detection of Sulfamethazine. JOURNAL OF COMPOSITES SCIENCE, v. 3, n. 2, p. 12-pg., 2019-06-01. (17/24274-3)