Preparation of functional mesoporous silica for intracellular controle release of bioactive species

• Grant number: 19/05467-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: May 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Marcos Augusto Bizeto
• Grantee: Marcos Augusto Bizeto
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Sang Won Han ; Vera Regina Leopoldo Constantino

Abstract

Intracellular therapies require the use of nanocarriers to transport, protect and control the release of the therapeutic species inside the cells. These nanocarriers are captured by the cells through endocytosis and are initially trapped into compartments called endosomes. This organelle is responsible for the digestion of the captured material and it is also the target site for many treatments. Depending on the type of treatment desired, the carrier may have different functions. In gene therapy, for example, it is necessary to promote a rapid endosome escape of the nanocarrier in order to avoid degradation of the genetic material before its release into the cytosol. To the treatment of inflammatory, degenerative and infectious diseases it is necessary the nanocarrier staying in this compartment for a longer period continuously releasing the drug. In the present study, nanocarriers based on mesoporous silica nanoparticles will be developed to perform such intracellular actions. Thus, prototypes of chemical and physical mechanisms to control the intracellular release of drugs and to promote the escape from the endosome will be developed. The chemical mechanisms to control the drug release will be based on the hydrolytic cleavage of covalent bonds catalyzed by the acidic pH of the endosome interior. Similar approach will be also used to release factors that cause the rupture of endosome membrane. The physical mechanisms will be based on the modification of the silica nanoparticles with superparamagnetic nanoparticles in order to use external magnetic fields to guide the nanocarrier and also to serve as stimulus for the release of the bioactive species based on magnetothermia phenomena. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIANCONI, LISTER P.; TAVIOT-GUEHO, CHRISTINE; CONSTANTINO, VERA REGINA L.; BIZETO, MARCOS AUGUSTO. Evaluation of the Structural Integrity of Layered Double Hydroxides and Mesoporous Silica During the Preparation of Heterostructures. Journal of the Brazilian Chemical Society, v. N/A, p. 8-pg., 2022-03-08. (19/05467-0, 16/50317-9)

CRAPINA, LAURA C.; HAN, SANG W.; BIZETO, MARCOS A.. Evaluation of colloidal properties of amino-functionalized mesoporous silica nanoparticles conjugated with the plasmid vector pEGFP-N3 and its influence on the pDNA delivery to HeLa cells. Microporous and Mesoporous Materials, v. 330, p. 9-pg., 2022-01-01. (15/20206-8, 19/05467-0)

LISTER P. BIANCONI; CHRISTINE TAVIOT-GUEHO; VERA REGINA L. CONSTANTINO; MARCOS AUGUSTO BIZETO. Evaluation of the Structural Integrity of Layered Double Hydroxides and Mesoporous Silica During the Preparation of Heterostructures. Journal of the Brazilian Chemical Society, v. 33, n. 10, p. 1211-1218, 2022-09-26. (19/05467-0)

FERREIRA, MARIANA MENDONCA; BIZETO, MARCOS AUGUSTO. Kinetic analysis of pH influence on the fluorescein release from chitosan-coated mesoporous silica nanoparticles. JOURNAL OF POROUS MATERIALS, v. 27, n. 4, APR 2020. (11/50318-1, 19/05467-0)