The bone response to exercise: a translational research program exploring clinical and mechanistic aspects

• Grant number: 19/05616-6
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: November 01, 2019 - October 31, 2024
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Convênio/Acordo: CONFAP ; Newton Fund, with FAPESP as a partner institution in Brazil ; UK Academies
• Principal Investigator: Eimear Bernadette Dolan
• Grantee: Eimear Bernadette Dolan
• Principal researcher abroad: Craig Sale
• Institution abroad: Nottingham Trent University, England
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 24/02583-8 - The influence of exercise training on chronotropic competence in individuals with Systemic Lupus Erythematosus prescribed with glucocorticoid pulse therapy., BP.MS; 23/06946-5 - The influence of low energy availability, with varying protein content, on the inflammatory response to an acute exercise bout., BP.MS; 22/09028-4 - The influence of PH on the bone biomarker response to exercise, BP.IC; + associated scholarships 22/09025-5 - Does acidosis moderate the bone metabolic response to acute exercise?, BP.MS; 21/02755-5 - Feasibility and Acceptability of a 6-month Supervised Exercise Training Intervention in a Group of Patients Undergoing High-Dose Glucocorticoid Therapy., BP.MS; 20/08637-1 - The influence of exercise capacity and dietary intake on bone mass and microarchitecture in rheumatic patients prescribed with high dose glucocorticoid therapy, BP.IC; 20/07860-9 - The influence of exercise training on bone health in patients taking high doses of glucocorticoids, BP.DD; 19/27758-7 - To investigate the relationship between pH and bone metabolic markers during a prolonged exercise test interspersed with high-intensity sprints, BP.IC; 19/26899-6 - The bone response to exercise a translational research program exploring clinical and mechanistic aspects, BP.JP - associated scholarships

Abstract

Exercise is an important means to optimize bone accrual and maintenance throughout the lifespan and to manage or treat conditions that cause bone loss or weakening. Additionally, the use of distinct exercise types and intensities provides unique opportunities to investigate the bone response to various metabolic and mechanical signals, thus providing an in vivo model to study bone physiology. Despite the importance of exercise as a model to understand basic bone physiology, and as a strategy to enhance bone health, little is known about the pathways that mediate the bone response to exercise, nor of the factors that moderate this response. Thus, the ability to develop and administer targeted and effective therapeutic exercise programs is limited. The proposed research program sits at the crucial interface between basic and applied science, and addresses important knowledge gaps in this topic area. As recommended by the UK Medical Research Council (along with many other research bodies), the original investigations described herein will be preceded by a statistical meta-analysis of all available data on the bone metabolic response to exercise (Study 1). Onward progression of knowledge in this clinically essential area is impeded by an incomplete understanding of current data, and this is mainly due to large heterogeneity in study design and quality. The proposed rigorously designed meta-analysis will combine high-quality data using multi-level modelling based on Bayesian principles, and will ensure that the proposed research program is grounded in the most up-to-date and highest-quality evidence available. Central to the proposed research program is a clinical RCT (Study 2), which will investigate the independent and combined influences of exercise training and protein supplementation on bone in a group of patients prescribed high-dose glucocorticoid therapy (GCT). This group are highly susceptible to bone loss and fracture, but the potential of exercise to protect bone when delivered as an adjunctive therapy to GCT has never been examined. Within this study, we will investigate important clinical outcomes (including bone mass and micro-architecture, along with physical function). To provide additional scientific rigor, we will also use bone biopsies and bone biomarkers to glean more in-depth mechanistic insight into the clinical results obtained. This clinical trial will be complemented by a number of in vivo laboratory-based mechanistic studies, within which we will examine the bone metabolic response to acidosis (Study 3) and exercise type (Study 4). Additionally, we will examine how important nutritional factors - namely energy availability and protein intake - moderate the bone response to exercise (Study 5).There is a critical need to understand better the specific factors that regulate the bone response to exercise, to ensure that lifestyle intervention interventions and recommendations are effective and fit-for-purpose. Collectively, this ambitious, novel and comprehensive research program will provide unique insights into the complex relationship between exercise and bone, along with the modifiable factors that mediate this response. Importantly, the data attained, in addition to its numerous direct therapeutic and clinical applications, will provide a foundation on which an extensive on-going research program can be built. In line with the objectives of the Young Investigator program, we are confident that the current proposal has all of the ingredients required to develop an innovative and impactful research line and group, that will serve as an internationally recognised centre of excellence for research investigating the bone response to exercise and nutrition, along with the clinical applications of this approach. (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAUNDERS, BRYAN; DE OLIVEIRA, LUANA FARIAS; DOLAN, EIMEAR; DURKALEC-MICHALSKI, KRZYSZTOF; MCNAUGHTON, LARS; ARTIOLI, GUILHERME GIANNINI; SWINTON, PAUL ALAN. Sodium bicarbonate supplementation and the female athlete: A brief commentary with small scale systematic review and meta-analysis. European Journal of Sport Science, FEB 2021. (19/25032-9, 16/50438-0, 19/26899-6, 19/05616-6)

DOLAN, E.; DUMAS, A.; KEANE, K. M.; BESTETTI, G.; FREITAS, L. H. M.; GUALANO, B.; KOHRT, W.; KELLEY, G. A.; PEREIRA, R. M. R.; SALE, C.; et al. The influence of acute exercise on bone biomarkers: protocol for a systematic review with meta-analysis. SYSTEMATIC REVIEWS, v. 9, n. 1, DEC 12 2020. (19/26899-6, 19/05616-6)

DOLAN, EIMEAR; ARTIOLI, GUILHERME G.; PEREIRA, ROSA MARIA R.; GUALANO, BRUNO. Muscular Atrophy and Sarcopenia in the Elderly: Is There a Role for Creatine Supplementation?. BIOMOLECULES, v. 9, n. 11, NOV 2019. (19/05616-6, 17/13552-2)

ESTEVES, GABRIEL P.; MAZZOLANI, BRUNA CARUSO; SMAIRA, FABIANA INFANTE; MENDES, ELIZABETH SILVA; DE OLIVEIRA, GABRIELA GUIMARAES; ROSCHEL, HAMILTON; GUALANO, BRUNO; PEREIRA, ROSA MARIA R.; DOLAN, EIMEAR. Nutritional recommendations for patients undergoing prolonged glucocorticoid therapy. RHEUMATOLOGY ADVANCES IN PRACTICE, v. 6, n. 2, p. 13-pg., 2022-05-06. (19/14820-6, 19/05616-6, 16/00006-7, 20/08637-1, 20/07860-9, 19/14819-8)

ELLIOTT-SALE, KIRSTY J.; MCNULTY, KELLY L.; ANSDELL, PAUL; GOODALL, STUART; HICKS, KIRSTY M.; THOMAS, KEVIN; SWINTON, PAUL A.; DOLAN, EIMEAR. The Effects of Oral Contraceptives on Exercise Performance in Women: A Systematic Review and Meta-analysis. SPORTS MEDICINE, v. 50, n. 10, JUL 2020. (19/05616-6)

MATTHEWS, JOSEPH J.; DOLAN, EIMEAR; SWINTON, PAUL A.; SANTOS, LIVIA; ARTIOLI, GUILHERME G.; TURNER, MARK D.; ELLIOTT-SALE, KIRSTY J.; SALE, CRAIG. The effect of carnosine or β-alanine supplementation on markers of glycaemic control and insulin resistance in human and animal studies: a protocol for a systematic review and meta-analysis. SYSTEMATIC REVIEWS, v. 9, n. 1, p. 6-pg., 2020-12-05. (19/05616-6, 14/11948-8, 19/25032-9, 19/26899-6)

BARRETO, GABRIEL; DE OLIVEIRA, LUANA FARIAS; SAITO, TIEMI; KLOSTERHOFF, RAFAEL; PERIM, PEDRO; DOLAN, EIMEAR; PEREIRA, ROSA MARIA R.; CAMPOS-FERRAZ, PATRICIA; LIMA, FERNANDA R.; SAUNDERS, BRYAN. Reduced Endurance Capacity and Suboptimal Energy Availability in Top-Level Female Cyclists. INTERNATIONAL JOURNAL OF SPORTS PHYSIOLOGY AND PERFORMANCE, v. 16, n. 8, p. 1194-1203, AUG 2021. (18/01594-5, 17/15314-1, 16/50438-0, 19/05616-6, 19/26899-6)

SCHWARTZ, JULIANO; OH, PAUL; TAKITO, MONICA Y.; SAUNDERS, BRYAN; DOLAN, EIMEAR; FRANCHINI, EMERSON; RHODES, RYAN E.; BREDIN, SHANNON S. D.; COELHO, JOSYE P.; DOS SANTOS, PEDRO; et al. Translation, Cultural Adaptation, and Reproducibility of the Physical Activity Readiness Questionnaire for Everyone (PAR-Q+): The Brazilian Portuguese Version. FRONTIERS IN CARDIOVASCULAR MEDICINE, v. 8, JUL 26 2021. (19/26899-6, 16/50438-0, 19/05616-6)

OLIVEIRA-JUNIOR, GERSIEL; PINTO, RAFAELA S.; SHIRLEY, MEGHAN K.; LONGMAN, DANIEL P.; KOEHLER, KARSTEN; SAUNDERS, BRYAN; ROSCHEL, HAMILTON; DOLAN, EIMEAR. The Skeletal Muscle Response to Energy Deficiency: A Life History Perspective. ADAPTIVE HUMAN BEHAVIOR AND PHYSIOLOGY, v. 8, n. 1, p. 16-pg., 2022-01-24. (19/05616-6, 19/26899-6, 20/07540-4, 16/50438-0)

DOLAN, EIMEAR; VARLEY, IAN; ACKERMAN, KATHRYN E.; PEREIRA, ROSA MARIA R.; ELLIOTT-SALE, KIRSTY JAYNE; SALE, CRAIG. The Bone Metabolic Response to Exercise and Nutrition. EXERCISE AND SPORT SCIENCES REVIEWS, v. 48, n. 2, p. 49-58, APR 2020. (19/05616-6)

PERIM, PEDRO; GOBBI, NATHAN; DUARTE, BRENO; FARIAS DE OLIVEIRA, LUANA; COSTA, LUIZ AUGUSTO RIANI; SALE, CRAIG; GUALANO, BRUNO; DOLAN, EIMEAR; SAUNDERS, BRYAN. Beta-alanine did not improve high-intensity performance throughout simulated road cycling. European Journal of Sport Science, v. 22, n. 8, p. 10-pg., 2021-06-16. (16/50438-0, 19/07497-4, 19/05616-6, 19/06140-5, 18/01594-5, 17/13552-2, 16/17391-0)

REZENDE, NATHALIA SAFFIOTI; BESTETTI, GIULIA CAZETTA; DE OLIVEIRA, LUANA FARIAS; MAZZOLANI, BRUNA CARUSO; SMAIRA, FABIANA INFANTE; DUMAS, ALINA; SWINTON, PAUL; SAUNDERS, BRYAN; DOLAN, EIMEAR. Dietary ?-Alanine Intake Assessed by Food Records Does Not Associate With Muscle Carnosine Content in Healthy, Active, Omnivorous Men and Women. INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, v. N/A, p. 8-pg., 2023-03-24. (19/05616-6, 21/06836-0, 22/09025-5, 19/26899-6)

SAUNDERS, BRYAN; DE OLIVEIRA, LUANA FARIAS; DOLAN, EIMEAR; DURKALEC-MICHALSKI, KRZYSZTOF; MCNAUGHTON, LARS; ARTIOLI, GUILHERME GIANNINI; SWINTON, PAUL ALAN. Sodium bicarbonate supplementation and the female athlete: A brief commentary with small scale systematic review and meta-analysis. European Journal of Sport Science, v. 22, n. 5, p. 10-pg., 2021-02-27. (19/25032-9, 16/50438-0, 19/26899-6, 19/05616-6)

CIVIL, RITA; DOLAN, EIMEAR; SWINTON, PAUL A.; SANTOS, LIVIA; VARLEY, IAN; ATHERTON, PHILIP J.; ELLIOTT-SALE, KIRSTY J.; SALE, CRAIG. P1NP and β-CTX-1 Responses to a Prolonged, Continuous Running Bout in Young Healthy Adult Males: A Systematic Review with Individual Participant Data Meta-analysis. SPORTS MEDICINE-OPEN, v. 9, n. 1, p. 16-pg., 2023-09-19. (19/05616-6, 19/26899-6)

PERIM, PEDRO; GOBBI, NATHAN; DUARTE, BRENO; FARIAS DE OLIVEIRA, LUANA; COSTA, LUIZ AUGUSTO RIANI; SALE, CRAIG; GUALANO, BRUNO; DOLAN, EIMEAR; SAUNDERS, BRYAN. Beta-alanine did not improve high-intensity performance throughout simulated road cycling. European Journal of Sport Science, JUN 2021. (19/07497-4, 17/13552-2, 18/01594-5, 19/06140-5, 16/17391-0, 16/50438-0, 19/05616-6)

MATTHEWS, JOSEPH J.; DOLAN, EIMEAR; SWINTON, PAUL A.; SANTOS, LIVIA; ARTIOLI, GUILHERME G.; TURNER, MARK D.; ELLIOTT-SALE, KIRSTY J.; SALE, CRAIG. The effect of carnosine or beta-alanine supplementation on markers of glycaemic control and insulin resistance in human and animal studies: a protocol for a systematic review and meta-analysis. SYSTEMATIC REVIEWS, v. 9, n. 1, DEC 5 2020. (14/11948-8, 19/26899-6, 19/25032-9, 19/05616-6)

ELLIOTT-SALE, KIRSTY J.; MCNULTY, KELLY L.; GOODALL, STUART; ANSDELL, PAUL; THOMAS, KEVIN; SWINTON, PAUL A.; DOLAN, EIMEAR; HICKS, KIRSTY M.. Reply to: Comment on: ``The Effects of Menstrual Cycle Phase on Exercise Performance in Eumenorrheic Women: A Systematic Review and Meta-Analysis{''} and ``The Effects of Oral Contraceptives on Exercise Performance in Women: A Systematic Review and Meta-analysis{''}. SPORTS MEDICINE, v. 51, n. 5, DEC 2020. (19/05616-6)

MATTHEWS, JOSEPH J.; DOLAN, EIMEAR; SWINTON, PAUL A.; SANTOS, LIVIA; ARTIOLI, GUILHERME G.; TURNER, MARK D.; ELLIOTT-SALE, KIRSTY J.; SALE, CRAIG. Effect of Carnosine or beta-Alanine Supplementation on Markers of Glycemic Control and Insulin Resistance in Humans and Animals: A Systematic Review and Meta-analysis. ADVANCES IN NUTRITION, v. 12, n. 6, p. 2216-2231, NOV 2021. (14/11948-8, 19/26899-6, 19/05616-6, 19/25032-9)

MCNULTY, KELLY LEE; ELLIOTT-SALE, KIRSTY JAYNE; DOLAN, EIMEAR; SWINTON, PAUL ALAN; ANSDELL, PAUL; GOODALL, STUART; THOMAS, KEVIN; HICKS, KIRSTY MARIE. The Effects of Menstrual Cycle Phase on Exercise Performance in Eumenorrheic Women: A Systematic Review and Meta-Analysis. SPORTS MEDICINE, v. 50, n. 10, JUL 2020. (19/05616-6)

ESTEVES, GABRIEL PERRI; SWINTON, PAUL; SALE, CRAIG; JAMES, RUTH M.; ARTIOLI, GUILHERME GIANNINI; ROSCHEL, HAMILTON; GUALANO, BRUNO; SAUNDERS, BRYAN; DOLAN, EIMEAR. Individual Participant Data Meta-Analysis Provides No Evidence of Intervention Response Variation in Individuals Supplementing With Beta-Alanine. INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM, v. 31, n. 4, p. 305-313, JUL 2021. (19/05616-6, 20/07860-9, 19/25032-9, 16/50438-0, 19/26899-6)

REZENDE, NATHALIA SAFFIOTI; SWINTON, PAUL; DE OLIVEIRA, LUANA FARIAS; DA SILVA, RAFAEL PIRES; DA EIRA SILVA, VINICIUS; NEMEZIO, KLEINER; YAMAGUCHI, GUILHERME; ARTIOLI, GUILHERME GIANNINI; GUALANO, BRUNO; SAUNDERS, BRYAN; et al. The Muscle Carnosine Response to Beta-Alanine Supplementation: A Systematic Review With Bayesian Individual and Aggregate Data E-Max Model and Meta-Analysis. FRONTIERS IN PHYSIOLOGY, v. 11, AUG 14 2020. (13/14746-4, 17/04973-4, 17/09635-0, 15/23176-2, 19/27758-7, 19/05616-6, 17/23688-9, 14/11948-8)

DOLAN, EIMEAR; WARREN, DANIEL E.; HARRIS, ROGER C.; SALE, CRAIG; GUALANO, BRUNO; SAUNDERS, BRYAN. Skeletal muscle histidine-containing dipeptide contents are increased in freshwater turtles (C. picta bellii) with cold-acclimation. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 262, DEC 2021. (19/26899-6, 17/13552-2, 16/50438-0, 19/05616-6)

SIECZKOWSKA, SOFIA MENDES; DE LIMA, ALISSON PADILHA; SWINTON, PAUL ALAN; DOLAN, EIMEAR; ROSCHEL, HAMILTON; GUALANO, BRUNO. Health Coaching Strategies for Weight Loss: A Systematic Review and Meta-Analysis. ADVANCES IN NUTRITION, v. 12, n. 4, p. 1449-1460, JUL 2021. (19/15231-4, 17/13552-2, 19/05616-6)

PERIM, PEDRO; MARTICORENA, FELIPE MIGUEL; RIBEIRO, FELIPE; BARRETO, GABRIEL; GOBBI, NATHAN; KERKSICK, CHAD; DOLAN, EIMEAR; SAUNDERS, BRYAN. Can the Skeletal Muscle Carnosine Response to Beta-Alanine Supplementation Be Optimized?. FRONTIERS IN NUTRITION, v. 6, AUG 27 2019. (18/01594-5, 17/15314-1, 17/04973-4, 16/50438-0, 19/05616-6, 18/19981-5)

ANDRE, HELOISA C. SANTO; ESTEVES, GABRIEL P.; BARRETO, GABRIEL. H. C.; LONGHINI, FERNANDO; DOLAN, EIMEAR; BENATTI, FABIANA B.. The Influence of n-3PUFA Supplementation on Muscle Strength, Mass, and Function: A Systematic Review and Meta-Analysis. ADVANCES IN NUTRITION, v. 14, n. 1, p. 13-pg., 2023-02-21. (19/17912-9, 19/05616-6, 20/12036-3, 20/07860-9, 19/26899-6, 20/02741-1, 21/12116-0)

ESTEVES, GABRIEL P.; SWINTON, PAUL; SALE, CRAIG; GUALANO, BRUNO; ROSCHEL, HAMILTON; DOLAN, EIMEAR. Use of factor analysis to model relationships between bone mass and physical, dietary, and metabolic factors in frail and pre-frail older adults. Journal of Applied Physiology, v. 135, n. 1, p. 8-pg., 2023-07-01. (17/13552-2, 19/05616-6, 20/07860-9, 19/26899-6)

ELLIOTT-SALE, KIRSTY J.; MCNULTY, KELLY L.; GOODALL, STUART; ANSDELL, PAUL; THOMAS, KEVIN; SWINTON, PAUL A.; DOLAN, EIMEAR; HICKS, KIRSTY M.. Reply to: Comment on: "The Effects of Menstrual Cycle Phase on Exercise Performance in Eumenorrheic Women: A Systematic Review and Meta-Analysis" and "The Effects of Oral Contraceptives on Exercise Performance in Women: A Systematic Review and Meta-analysis". SPORTS MEDICINE, v. 51, n. 5, p. 3-pg., 2020-12-17. (19/05616-6)