Anti-inflammatory effect of triamcinolone and platelet-rich plasma on Lateral elbow Epicondylitis cells

• Grant number: 19/13468-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2020
• End date: September 30, 2022
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Silvana Artioli Schellini
• Grantee: Silvana Artioli Schellini
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Alexandre Todorovic Fabro ; Claudia Aparecida Rainho ; Magda Massae Hata Viveiros ; Márcia Guimarães da Silva ; Márcio Eduardo de Melo Viveiros

Abstract

Purpose:* To culture and to characterize lateral elbow epicondylitis cells with the markers: collagen type I, II, III, scleraxis, osteocalcin, alpha muscle actin and vimentin by immunohistochemistry.* To evaluate the basal production index of inflammatory cytokines IL-1², IL-6, IL-8, IL-10, TNF-±, FGF, VEGF and GM-CSF by lateral epicondylitis cells in cell culture.* To compare the in vitro anti-inflammatory effect on the production of inflammatory cytokines on lateral epicondylitis cells after exposure to triamcinolone and PRP.Methods:An in vitro study will be performed with primary culture of lateral epicondylitis elbow cells. The cultured cells will initially be characterized by the immunohistochemical marking with collagen type I, II and III, scleraxis, osteocalcin, alpha muscle actin and vimentin, in order to elucidate the cellular composition of the lateral epicondylitis in tenocytes, osteoblasts or fibroblasts.Subsequently, a MTT viability assay will be performed after exposure to different concentrations of triamcinolone acetate, in order to establish the concentrations and time points to be studied. Cells of third passage will be exposed in triplicates to triamcinolone and platelet-rich plasma for comparison of their anti-inflammatory effects, accessing the production of the cytokines IL-1², IL-6, IL- 8, IL-10, TNF-±, FGF, VEGF and GM-CSF by ELISA. The study of soluble SGP-130 receptors will indicate whether IL-6 is exerting an anti-or pro-inflammatory effect. The data will be submitted to statistical analysis by parametric test not matched with Welch's correction. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELO VIVEIROS, MARCIO EDUARDO; HATA VIVEIROS, MAGDA MASSAE; DA SILVA, MARCIA GUIMARAES; RAINHO, CLAUDIA APARECIDA; SCHELLINI, SILVANA ARTIOLI. n vitro effect of triamcinolone and platelet-rich plasma on cytokine levels of elbow lateral epicondylitis-derived cell. JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, v. 17, n. 1, FEB 15 2022. (19/13468-7)